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Avian Antibodies Can Eliminate Interference Due To Complement Activation In ELISA
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
2002 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 106, no 3, 189-195 p.Article in journal (Refereed) Published
Abstract [en]

Antibodies derived from egg yolk offer many advantages over mammalian antibodies in several aspects. Chicken antibodies do not activate the human complement system and are sometimes a more suitable choice in designing solid-phase immunometric assays than mammalian antibodies. The material often recommended for immunological assays is serum. A freshly drawn serum sample contains an active complement system, which is inactivated during storage. Mammalian antibodies used in most immunological assays may activate the human complement system. Activated complement components will bind to the antibodies thereby partly block the antibody binding epitopes. We show that an active complement system in undiluted samples reduce the absorbance values by approximately 50 % when using goat antibodies but not when using chicken antibodies. This difference will cause erroneous test results that will vary depending on the handling of the samples. Chicken antibodies can be used to eliminate this interference problem.

Place, publisher, year, edition, pages
2002. Vol. 106, no 3, 189-195 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-89637PubMedID: 12166511OAI: oai:DiVA.org:uu-89637DiVA: diva2:161293
Available from: 2002-02-11 Created: 2002-02-11 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Avian IgY antibody: In vitro and in vivo
Open this publication in new window or tab >>Avian IgY antibody: In vitro and in vivo
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Immunoglobulin Y (IgY) is the major antibody found in eggs from chicken (Gallus domesticus). IgY can be used as an alternative to mammalian antibodies normally used in research, and its use in immunotherapy has recently been proposed. Compared to mammalian antibodies, IgY possesses several biochemical advantages and its simple purification from egg yolk prevents a stressful moment in animal handling, as no bleeding is necessary.

Small amount of antigen (1 mg) can be used to elicit an immune response in chickens and there are low intra-individual differences regarding antibody concentration found in yolk. By studying two chicken breeds and their cross, a genetic correlation was shown regarding the IgY concentration, which implies a possibility by breeding to increase IgY concentrations. By using IgY instead of goat antibody as capture antibody in ELISA, it is possible reduce interferences by complement activation. After oral administration of IgY to healthy volunteers, IgY activity was present in saliva 8 hours later, indicating a protective effect. This effect has been studied in an open clinical trial with cystic fibrosis patients. Specific IgY against Pseudomonas aeruginosa given orally prolongs the time of intermittent colonization by six months, decrease the number of positive colonizations and might be a useful complement to antibiotic treatment. Immunoglobulin therapy may diminish the development of antibiotic resistant microorganisms. The use of immunoglobulin therapy broadens the arsenal available to combat pathogens in medicine and IgY is a promising candidate, both as an alternative to antibiotics and as a useful tool in research and diagnostics.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2002. 53 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1119
Keyword
Medical sciences, Antigen production, chicken, cystic fibrosis, egg, ELISA, IgY, immunotherapy, Pseudomonas aeruginosa, yolk, MEDICIN OCH VÅRD
National Category
Medical and Health Sciences
Research subject
Clinical Chemistry
Identifiers
urn:nbn:se:uu:diva-1710 (URN)91-554-5227-2 (ISBN)
Public defence
2002-03-06, Rosénssalen, Ing 95, Akademiska sjukhuset, Uppsala, 09:15
Opponent
Available from: 2002-02-11 Created: 2002-02-11Bibliographically approved

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Carlander, DavidLarsson, Anders

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