Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator
1999 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 27, no 6, 1552-1554 p.Article in journal (Refereed) Published
Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome entry site of the encephalomyocarditis virus, to enable linkage of the Cre recombinase or rtTA trans-activator to 3' untranslated ends of endogenous genes. Here we report that these constructs function effectively in COS cells. The data suggest that these cassettes will be appropriate for 3' targeting of mouse genes.
Place, publisher, year, edition, pages
1999. Vol. 27, no 6, 1552-1554 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-89676DOI: 10.1093/nar/27.6.1552PubMedID: 10037820OAI: oai:DiVA.org:uu-89676DiVA: diva2:161354