High yield direct 76Br-bromination of monoclonal antibodies using Cloramine-T
1999 (English)In: Nuclear Medicine and Biology, ISSN 0969-8051, Vol. 26, no 8, 923-929 p.Article in journal (Refereed) Published
Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.
Place, publisher, year, edition, pages
1999. Vol. 26, no 8, 923-929 p.
76Br, Radiobromination, PET, CAT-labeling, Monoclonal antibodies, A33
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-89743DOI: 10.1016/S0969-8051(99)00076-1PubMedID: 10708306OAI: oai:DiVA.org:uu-89743DiVA: diva2:161472