Fractionation protocol for the isolation of polypeptides from plant biomass
1998 (English)In: Journal of natural products (Print), ISSN 0163-3864, E-ISSN 1520-6025, Vol. 61, no 1, 77-81 p.Article in journal (Refereed) Published
A fractionation protocol for the isolation of a highly purified polypeptide fraction from plant biomass is described. The procedure dereplicates ubiquitous substance classes known to interfere with bioassays often used in natural product-based drug discovery programs. The protocol involves pre-extraction with dichloromethane, extraction with ethanol (50%), removal of tannins with polyamide, removal of low-molecular-weight components with size-exclusion chromatography over Sephadex G-10, and final removal of salts and polysaccharides with solid-phase extraction using reversed-phase cartridges. The method has been applied to the aerial parts of Viola arvensis, resulting in the isolation of a peptide fraction that on further separation yielded a novel 29-residue macrocyclic polypeptide named varv peptide A, cyclo(-TCVGGTCNTPGCSCSWPVCTRNGLPVCGE-).
Place, publisher, year, edition, pages
1998. Vol. 61, no 1, 77-81 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-89791DOI: 10.1021/np970342rPubMedID: 9548831OAI: oai:DiVA.org:uu-89791DiVA: diva2:161545