Electron Capture Dissociation of Substance-P using a Commercially Available Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
1999 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 13, no 6, 474-477 p.Article in journal (Refereed) Published
Electron capture dissociation of the peptide Substance P is reported for the first time, with an unmodified, commercially available Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The fragmentation pattern is compared with that obtained with collisionally induced dissociation of the ions in the electrospray ion source, and note that electron capture dissociation gives a more easily interpreted spectrum, showing mainly C-fragments. With the exception of the proline residues, which require cleavage of two chemical bonds, we observe all C-fragmental we find the bias voltage of the electron gun not to be very critical.
Place, publisher, year, edition, pages
1999. Vol. 13, no 6, 474-477 p.
IdentifiersURN: urn:nbn:se:uu:diva-89816DOI: 10.1002/(SICI)1097-0231(19990330)13:6<474::AID-RCM505>3.0.CO;2-1PubMedID: 10204243OAI: oai:DiVA.org:uu-89816DiVA: diva2:161601