Prediction of chromatographic retention and protein identification in liquid chromatography/mass spectrometry
2002 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 74, no 22, 5826-5830 p.Article in journal (Refereed) Published
Liquid chromatography coupled on- or off-line with mass spectrometry is rapidly advancing as a tool in proteomics capable of dealing with the inherent complexity in biology and complementing conventional approaches based on two-dimensional gel electrophoresis. Proteins can be identified by proteolytic digestion and peptide mass fingerprinting or by searching databases using short-sequence tags generated by tandem mass spectrometry. This paper shows that information on the chromatographic behavior of peptides can assist protein identification by peptide mass fingerprinting in liquid chromatography/mass spectrometry. This additional information is significant and already available at no extra experimental cost.
Place, publisher, year, edition, pages
2002. Vol. 74, no 22, 5826-5830 p.
IdentifiersURN: urn:nbn:se:uu:diva-89821DOI: 10.1021/ac0256890PubMedID: 12463368OAI: oai:DiVA.org:uu-89821DiVA: diva2:161606