uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Detection of immunoglobulin heavy chain gene rearrangement with PCR for MRD analysis in lymphoproliferative disorders
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Genetics and Pathology.
1998 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Immunoglobulin heavy chain (IGH) gene rearrangement occurs during early B-lymphocyte differentiation, assembling the different IGH gene segments to a functional gene, which can serve as a marker for study of lineage association and detection of Minimal Residual Disease (MRD) in clonal diseases deriving from B-lymphocytes or their early differentiation stages. Use of a molecular marker for the leukemic cells could help improve treatment by monitoring therapeutic efficacy, predicting relapse, and identifying very small amounts of tumour cells contaminating autografts after purging or enrichment of stem cells.

This work focused on the task of improving the specificity of clonality and MRD tests by developing simple laboratory methods based on Polymerase Chain Reaction (PCR) with which to distinguish monoclonal from polyclonal cells. These methods were compared with conventional histopathological and immunocytological techniques in a retrospective study concerning the possibility of predicting recurrence of childhood pre-B Acute Lymphoblastic Leukemia (ALL). Clonal evolution in childhood pre-B ALL was also studied by comparing the rearrangements at diagnosis versus at relapse. A highly sensitive clonospecific PCR-method was utilized to detect small amounts of monoclonal cells before and after stem cell purging in mobilized peripheral blood stem cells from multiple myeloma patients.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 1998. , 58 p.
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 802
Keyword [en]
Genetics, Minimal residual disease, Immunoglobulin gene rearrangement, PCR, Leukemia, Lymphoma, Myeloma
Keyword [sv]
National Category
Medical Genetics
Research subject
URN: urn:nbn:se:uu:diva-242ISBN: 91-554-4338-9OAI: oai:DiVA.org:uu-242DiVA: diva2:161853
Public defence
1998-12-18, Fåhraeussalen, Dep., of Genetics and Pathology, Uppsala University, Uppsala, 09:00
Available from: 1998-11-27 Created: 1998-11-27Bibliographically approved

Open Access in DiVA

No full text
Buy this publication >>

By organisation
Department of Genetics and Pathology
Medical Genetics

Search outside of DiVA

GoogleGoogle Scholar

Total: 70 hits
ReferencesLink to record
Permanent link

Direct link