Improved lectin-mediated immobilization of human red blood cells in superporous agarose beads
2003 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 784, no 1, 203-208 p.Article in journal (Refereed) Published
A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.
Place, publisher, year, edition, pages
2003. Vol. 784, no 1, 203-208 p.
Wheat germ agglutinin, Cytochalasin B, GLUT1, Immobilization, Superporous agarose
IdentifiersURN: urn:nbn:se:uu:diva-90004DOI: 10.1016/S1570-0232(02)00758-4PubMedID: 12504199OAI: oai:DiVA.org:uu-90004DiVA: diva2:162004