Separation of L-DOPA and four metabolites in plasma using a porous graphitic carbon column in capillary liquid chromatography
2002 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 55, no 1-2, 39-42 p.Article in journal (Refereed) Published
Porous graphitic carbon has been successfully used for the separation of L-DOPA and four metabolites (dopamine, 3,4-dihydroxy-phenyl-acetic acid, homovanillic acid and 3-O-methyl-L-DOPA) and with a volatile mobile phase of relatively high percentage organic modifier (methanol-ammonium formate pH 2.9, 60∶40, v/v). The substances were analysed in plasma. Ultrafiltration was used to remove the proteins from the sample and a coupled column system to enrich and perform on-line sample clean up. Both precolumn and separation column of i. d. 0.2 mm were packed with porous graphitic carbon.
Place, publisher, year, edition, pages
2002. Vol. 55, no 1-2, 39-42 p.
IdentifiersURN: urn:nbn:se:uu:diva-90137DOI: 10.1007/BF02492312OAI: oai:DiVA.org:uu-90137DiVA: diva2:162363