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Separation of L-DOPA and four metabolites in plasma using a porous graphitic carbon column in capillary liquid chromatography
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
2002 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 55, no 1-2, p. 39-42Article in journal (Refereed) Published
Abstract [en]

Porous graphitic carbon has been successfully used for the separation of L-DOPA and four metabolites (dopamine, 3,4-dihydroxy-phenyl-acetic acid, homovanillic acid and 3-O-methyl-L-DOPA) and with a volatile mobile phase of relatively high percentage organic modifier (methanol-ammonium formate pH 2.9, 60∶40, v/v). The substances were analysed in plasma. Ultrafiltration was used to remove the proteins from the sample and a coupled column system to enrich and perform on-line sample clean up. Both precolumn and separation column of i. d. 0.2 mm were packed with porous graphitic carbon.

Place, publisher, year, edition, pages
2002. Vol. 55, no 1-2, p. 39-42
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-90137DOI: 10.1007/BF02492312OAI: oai:DiVA.org:uu-90137DiVA, id: diva2:162363
Available from: 2003-02-25 Created: 2003-02-25 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Aspects of Porous Graphitic Carbon as Packing Material in Capillary Liquid Chromatography
Open this publication in new window or tab >>Aspects of Porous Graphitic Carbon as Packing Material in Capillary Liquid Chromatography
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, porous graphitic carbon (PGC) has been used as packing material in packed capillary liquid chromatography. The unique chromatographic properties of PGC has been studied in some detail and applied to different analytical challenges using both electrospray ionization-mass spectrometry (ESI-MS) and ultra violet (UV) absorbance detection.

The crucial importance of disengaging the conductive PGC chromatographic separation media from the high voltage mass spectrometric interface has been shown. In the absence of a grounded point between the column and ESI emitter, a current through the column was present, and changed retention behaviors for 3-O-methyl-DOPA and tyrosine were observed. An alteration of the chromatographic properties was also seen when PGC was chemically oxidized with permanganate, possibly due to an oxidation of the few surface groups present on the PGC material.

The dynamic adsorption of the chiral selector lasalocid onto the PGC support resulted in a useful and stable chiral stationary phase. Extraordinary enantioselectivity was observed for 1-(1-naphthyl)ethylamine, and enantioseparation was also achieved for other amines, amino acids, acids and alcohols.

Finally, a new strategy for separation of small biologically active compounds in plasma and brain tissue has been developed. With PGC as stationary phase it was possible to utilize a mobile phase of high content of organic modifier, without the addition of ion-pairing agents, and still selectively separate the analytes.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2003. p. 44
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 805
Keywords
Analytical chemistry, PGC, porous graphitic carbon, stationary phase, packing material, LC, liquid chromatography, miniaturized, chiral, enantiomer, ESI, electrospray ionization, mass spectrometry, polar compounds, L-DOPA, dopamine, biological samples, biological matrix, Analytisk kemi, PGC, poröst grafitiserat kol, stationärfas, packningsmaterial, LC, vätskekromatografi, miniatyriserade system, kiral, enantiomer, ESI, electrospray jonisation, mass spektrometri, polära föreningar, L-DOPA, dopmamine, biologiska prover, biologisk matris
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-3306 (URN)91-554-5532-8 (ISBN)
Public defence
2003-03-20, BMC, B21, Uppsala, 10:15
Opponent
Available from: 2003-02-25 Created: 2003-02-25 Last updated: 2013-06-13Bibliographically approved

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