Analysis of catecholamines and related substances using porous graphitic carbon as separation media in liquid chromatography-tandem mass spectrometry
2004 (English)In: Journal of Chromotography B, Vol. 801, no 2, 323-329 p.Article in journal (Refereed) Published
Capillary porous graphitic carbon (PGC) columns have been utilized for separation of several catecholamines and related compounds (i.e. l-tyrosine, l-DOPA, 3-O-methyl-DOPA, dopamine, 3,4-dihydroxy-phenyl-acetic acid (DOPAC), homovanillic acid, noradrenaline, vanillomandelic acid and adrenaline) on-line with electrospray ionization tandem mass spectrometry (ESI–MS/MS). The use of a mobile phase without ion-pairing agents and with high content of organic modifier facilitated the coupling to the selective and sensitive mass spectrometric detection. Minimum detectable sample concentration (MDC sample) for noradrenaline, dopamine and l-tyrosine in a standard solution was estimated to 3, 10 and 30 nM, respectively (3 S/N corresponds to MDQ for l-tyrosine of approximately 8×10−14 mol). The developed strategy was applied for analysis of brain tissue, i.e. a substantia nigra (ns) sample.
Place, publisher, year, edition, pages
2004. Vol. 801, no 2, 323-329 p.
Cateholamine, Porous graphitic carbon, L-DOPA, Dopamine, Polar analytes
IdentifiersURN: urn:nbn:se:uu:diva-90138DOI: 10.1016/j.jchromb.2003.11.036ISI: 000188741000021OAI: oai:DiVA.org:uu-90138DiVA: diva2:162364