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Analysis of catecholamines and related substances using porous graphitic carbon as separation media in liquid chromatography-tandem mass spectrometry
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
2004 (English)In: Journal of Chromotography B, Vol. 801, no 2, 323-329 p.Article in journal (Refereed) Published
Abstract [en]

Capillary porous graphitic carbon (PGC) columns have been utilized for separation of several catecholamines and related compounds (i.e. l-tyrosine, l-DOPA, 3-O-methyl-DOPA, dopamine, 3,4-dihydroxy-phenyl-acetic acid (DOPAC), homovanillic acid, noradrenaline, vanillomandelic acid and adrenaline) on-line with electrospray ionization tandem mass spectrometry (ESI–MS/MS). The use of a mobile phase without ion-pairing agents and with high content of organic modifier facilitated the coupling to the selective and sensitive mass spectrometric detection. Minimum detectable sample concentration (MDC sample) for noradrenaline, dopamine and l-tyrosine in a standard solution was estimated to 3, 10 and 30 nM, respectively (3 S/N corresponds to MDQ for l-tyrosine of approximately 8×10−14 mol). The developed strategy was applied for analysis of brain tissue, i.e. a substantia nigra (ns) sample.

Place, publisher, year, edition, pages
2004. Vol. 801, no 2, 323-329 p.
Keyword [en]
Cateholamine, Porous graphitic carbon, L-DOPA, Dopamine, Polar analytes
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-90138DOI: 10.1016/j.jchromb.2003.11.036ISI: 000188741000021OAI: oai:DiVA.org:uu-90138DiVA: diva2:162364
Available from: 2003-02-25 Created: 2003-02-25 Last updated: 2012-10-26Bibliographically approved
In thesis
1. Aspects of Porous Graphitic Carbon as Packing Material in Capillary Liquid Chromatography
Open this publication in new window or tab >>Aspects of Porous Graphitic Carbon as Packing Material in Capillary Liquid Chromatography
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, porous graphitic carbon (PGC) has been used as packing material in packed capillary liquid chromatography. The unique chromatographic properties of PGC has been studied in some detail and applied to different analytical challenges using both electrospray ionization-mass spectrometry (ESI-MS) and ultra violet (UV) absorbance detection.

The crucial importance of disengaging the conductive PGC chromatographic separation media from the high voltage mass spectrometric interface has been shown. In the absence of a grounded point between the column and ESI emitter, a current through the column was present, and changed retention behaviors for 3-O-methyl-DOPA and tyrosine were observed. An alteration of the chromatographic properties was also seen when PGC was chemically oxidized with permanganate, possibly due to an oxidation of the few surface groups present on the PGC material.

The dynamic adsorption of the chiral selector lasalocid onto the PGC support resulted in a useful and stable chiral stationary phase. Extraordinary enantioselectivity was observed for 1-(1-naphthyl)ethylamine, and enantioseparation was also achieved for other amines, amino acids, acids and alcohols.

Finally, a new strategy for separation of small biologically active compounds in plasma and brain tissue has been developed. With PGC as stationary phase it was possible to utilize a mobile phase of high content of organic modifier, without the addition of ion-pairing agents, and still selectively separate the analytes.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2003. 44 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 805
Keyword
Analytical chemistry, PGC, porous graphitic carbon, stationary phase, packing material, LC, liquid chromatography, miniaturized, chiral, enantiomer, ESI, electrospray ionization, mass spectrometry, polar compounds, L-DOPA, dopamine, biological samples, biological matrix, Analytisk kemi, PGC, poröst grafitiserat kol, stationärfas, packningsmaterial, LC, vätskekromatografi, miniatyriserade system, kiral, enantiomer, ESI, electrospray jonisation, mass spektrometri, polära föreningar, L-DOPA, dopmamine, biologiska prover, biologisk matris
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-3306 (URN)91-554-5532-8 (ISBN)
Public defence
2003-03-20, BMC, B21, Uppsala, 10:15
Opponent
Available from: 2003-02-25 Created: 2003-02-25 Last updated: 2013-06-13Bibliographically approved

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Sjöberg, Per J. R.Bergquist, Jonas

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