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Intraluminal acid and gastric mucosal integrity;the importance of blood-borne bicarbonate
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2001 In: Am J Physiol Gastrointest Liver Physiol, Vol. 280, G121-G129 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2001. Vol. 280, G121-G129 p.
URN: urn:nbn:se:uu:diva-90225OAI: oai:DiVA.org:uu-90225DiVA: diva2:162511
Available from: 2003-04-15 Created: 2003-04-15 Last updated: 2012-12-14
In thesis
1. Acid transport through gastric mucus: A study in vivo in rats and mice
Open this publication in new window or tab >>Acid transport through gastric mucus: A study in vivo in rats and mice
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The gastric mucosa is frequently exposed to endogenously secreted hydrochloric acid of high acidity. Gastric mucosal defense mechanisms are arranged at different levels of the gastric mucosa and must work in unison to maintain its integrity.

In this thesis, several mechanisms underlying gastric mucosal resistance to strong acid were investigated in anesthetized rats and mice. The main findings were as follows:

Only when acid secretion occurred did the pH gradient in the mucus gel withstand back-diffusion of luminal acid (100 mM or 155 mM HCl), and keep the juxtamucosal pH (pHjm) neutral. Thus, when no acid secretion occurred and the luminal pH was 0.8-1, the pH gradient was destroyed.

Bicarbonate ions, produced concomitant with hydrogen ions in the parietal cells during acid secretion and blood-borne to the surface epithelium, were carried transepithelially through a DIDS-sensitive transport.

Prostaglandin-dependent bicarbonate secretion seemed to be less important in maintaining a neutral pHjm.

Removal of the loosely adherent mucus layer did not influence the maintenance of the pHjm. Hence, only the firmly adherent mucus gel layer, approximately 80µm thick, seemed to be important for the pHjm.

Staining of the mucus gel with a pH-sensitive dye revealed that secreted acid penetrated the mucus gel from the crypt openings toward the gastric lumen only in restricted paths (channels). One crypt opening was attached to one channel, and the channel was irreversibly formed during acid secretion.

Gastric mucosal blood flow increased on application of strong luminal acid (155 mM HCl). This acid-induced hyperemia involved the inducible but not the neural isoform of nitric oxide synthase. These results suggest a novel role for iNOS in gastric mucosal protection and indicate that iNOS is constitutively expressed in the gastric mucosa.

It is concluded that a pH gradient in the gastric mucus gel can be maintained during ongoing acid secretion, since the acid penetrates the mucus only in restricted channels and bicarbonate is carried from the blood to the lumen via a DIDS-sensitive transporter.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2003. 64 p.
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1239
Physiology, gastric acid, pH-sensitive microelectrode, mucus, mucosal blood flow, nitric oxide, intravital microscopy, laser Doppler flowmetry, Fysiologi
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Research subject
urn:nbn:se:uu:diva-3368 (URN)91-554-5577-8 (ISBN)
Public defence
2003-05-09, B22, BMC, Uppsala, 13:15
Available from: 2003-04-15 Created: 2003-04-15Bibliographically approved

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