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Acute elevations of medium- and long-chain fatty acids have different impacts on endothelium-dependent vasodilation in humans
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Akut- och internmedicin)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences. (Clinical nutrition)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences. (Geriatrics)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences. (Clinical nutrition)
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2003 (English)In: Lipids, ISSN 0024-4201, E-ISSN 1558-9307, Vol. 38, no 1, 15-19 p.Article in journal (Refereed) Published
Abstract [en]

It has previously been shown that acute elevation of long-chain fatty acids (LCFA) impairs endothelium-dependent vasodilation (EDV) in humans. In this study, we tested the hypothesis that an elevation of both medium-chain fatty acids (MCFA) and LCFA affects the endothelium differently from LCFA elevation alone. Ten healthy volunteers received an intravenous infusion of Structolipid (structured TG, MCFA/LCFA ratio 1:1) and heparin for 2 h, while another 10 subjects received an infusion of Intralipid (LCFA only) and heparin. EDV and endothelium-independent vasodilation (EIDV) were studied in the forearm after local administration of methacholine chloride (2 and 4 microg/min) and sodium nitroprusside (5 and 10 microg/min). Forearm blood flow was determined by venous occlusion plethysmography. Intralipid and heparin increased circulating FA levels from 0.2 +/- 0.1 to 1.4 +/- 0.5 mmol/L (P < 0.001) and reduced EDV by 20% (P < 0.01). Although Structolipid and heparin increased circulating FA levels to a similar extent (from 0.4 +/- 0.1 to 1.8 +/- 0.4 mmol/L after 2 h), EDV was not significantly changed. EIDV increased slightly during both interventions (P < 0.05). In conclusion, an acute elevation of LCFA attenuated EDV, whereas an elevation of both MCFA and LCFA did not influence EDV. Thus, FA composition seems to be of importance for EDV in healthy humans.

Place, publisher, year, edition, pages
2003. Vol. 38, no 1, 15-19 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-90412DOI: 10.1007/s11745-003-1025-9PubMedID: 12669814OAI: oai:DiVA.org:uu-90412DiVA: diva2:162756
Available from: 2003-05-06 Created: 2003-05-06 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Lipids and Endothelium-Dependent Vasodilation
Open this publication in new window or tab >>Lipids and Endothelium-Dependent Vasodilation
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Lipider och endotelberoende vasodilatation
Abstract [en]

Impaired endothelium-dependent vasodilation (EDV) is associated with atherosclerotic cardiovascular disease as well as several of its risk factors.

The aim of the present thesis was to investigate how lipids influence EDV in the vascular bed of the human forearm.

Apolipoprotein B was inversely associated with both EDV and endothelium-independent vasodilation (EIDV) in healthy subjects aged 20-69 years. HDL cholesterol was associated with the EDV to EIDV ratio (EFI). Small LDL particles and antibodies against oxidized LDL were not associated with endothelial vasodilatory function.

The EFI in young, healthy subjects was positively associated with alpha-linolenic acid proportion, but inversely associated with myristic acid in men only. Eicosapentaenoic acid was positively associated with EDV, whereas dihomo-gamma-linolenic acid was inversely associated with both EDV and EIDV in men.

Acute elevation of long-chain fatty acids with Intralipid®/heparin infusion in young, healthy subjects impaired EDV after 2 h. This impairment could be prevented by co-infusing vitamin C, diclophenac or L-arginine. Acute elevation of both medium-chain and long-chain fatty acids during Structolipid®/heparin infusion did not impair EDV.

An ordinary meal (34 E% fat) transiently attenuated EDV at 1 hour. No attenuation in EDV was observed after meals containing 20 and 3 E% fat.

These findings show that the endothelial vasodilatory function is associated with fatty acid profile in serum in the fasting state and during acute fatty acid elevation, as well as with apolipoprotein B and HDL cholesterol. Furthermore, lowering dietary fat content to 20 E% or less preserves endothelial vasodilatory function and might therefore protect against atherosclerosis.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2003. 78 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1263
Keyword
Medicine, Atherosclerosis, endothelium, vasodilation, nitric oxide, fatty acid, meal, diet, lipoprotein, cyclooxygenase, diclophenac, L-arginine, LDL, human, Medicin
National Category
Dermatology and Venereal Diseases
Research subject
Internal Medicine
Identifiers
urn:nbn:se:uu:diva-3424 (URN)91-554-5633-2 (ISBN)
Public defence
2003-05-26, Aulan, Thoraxhuset - ingång 50, Akademiska sjukhuset, Uppsala, 09:15
Opponent
Supervisors
Available from: 2003-05-06 Created: 2003-05-06 Last updated: 2010-10-21Bibliographically approved

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Steer, PeterBasu, SamarVessby, BengtBerne, ChristianLind, Lars

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