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The effect of a mixed meal on endothelium-dependent vasodilation is dependent on fat content in healthy humans
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2003 (English)In: Clinical Science, ISSN 0143-5221, E-ISSN 1470-8736, Vol. 105, no 1, 81-87 p.Article in journal (Refereed) Published
Abstract [en]

Impaired endothelium-dependent vasodilation (EDV) is an early marker of atherosclerosis. The aim of the present study was to investigate how meals with different fat contents influence endothelial vasodilatory function. A total of 26 young, healthy men and women aged 20-30 years ingested an ordinary Western meal [34 energy% (E%) fat, n =10], or isocaloric meals with low-fat (20 E%, n =8), or minimal-fat (3 E%, n =8) content. EDV was assessed as forearm blood flow (FBF) during local administration of 4 microg/min methacholine chloride (Mch-FBF) and endothelium-independent vasodilation as FBF during administration of 10 microg/min sodium nitroprusside (SNP-FBF) at baseline and 1 and 2 h after each meal. FBF was determined by venous occlusion plethysmography. An endothelial function index (EFI) was calculated as the Mch-FBF/SNP-FBF ratio. Both Mch-FBF and the EFI were decreased at 1 h after the 34 E% fat meal ( P <0.01 and P <0.05 respectively), but approached fasting levels after 2 h. Mch-FBF and EFI did not change significantly in the group consuming the 20 E% fat meal, but increased in the 3 E% fat group ( P <0.01 and P <0.05 compared with baseline for Mch-FBF and EFI respectively). SNP-FBF was not significantly affected by any of the meals. In conclusion, low-fat meals did not attenuate EDV, in contrast with an ordinary Western meal, which transiently impaired EDV. Our findings indicate that a dietary fat content of 20 E% or less might be beneficial to endothelial vasodilatory function.

Place, publisher, year, edition, pages
2003. Vol. 105, no 1, 81-87 p.
Keyword [en]
diet, endothelium, fatty acid, insulin, meal
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-90413PubMedID: 12625832OAI: oai:DiVA.org:uu-90413DiVA: diva2:162757
Available from: 2003-05-06 Created: 2003-05-06 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Lipids and Endothelium-Dependent Vasodilation
Open this publication in new window or tab >>Lipids and Endothelium-Dependent Vasodilation
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Lipider och endotelberoende vasodilatation
Abstract [en]

Impaired endothelium-dependent vasodilation (EDV) is associated with atherosclerotic cardiovascular disease as well as several of its risk factors.

The aim of the present thesis was to investigate how lipids influence EDV in the vascular bed of the human forearm.

Apolipoprotein B was inversely associated with both EDV and endothelium-independent vasodilation (EIDV) in healthy subjects aged 20-69 years. HDL cholesterol was associated with the EDV to EIDV ratio (EFI). Small LDL particles and antibodies against oxidized LDL were not associated with endothelial vasodilatory function.

The EFI in young, healthy subjects was positively associated with alpha-linolenic acid proportion, but inversely associated with myristic acid in men only. Eicosapentaenoic acid was positively associated with EDV, whereas dihomo-gamma-linolenic acid was inversely associated with both EDV and EIDV in men.

Acute elevation of long-chain fatty acids with Intralipid®/heparin infusion in young, healthy subjects impaired EDV after 2 h. This impairment could be prevented by co-infusing vitamin C, diclophenac or L-arginine. Acute elevation of both medium-chain and long-chain fatty acids during Structolipid®/heparin infusion did not impair EDV.

An ordinary meal (34 E% fat) transiently attenuated EDV at 1 hour. No attenuation in EDV was observed after meals containing 20 and 3 E% fat.

These findings show that the endothelial vasodilatory function is associated with fatty acid profile in serum in the fasting state and during acute fatty acid elevation, as well as with apolipoprotein B and HDL cholesterol. Furthermore, lowering dietary fat content to 20 E% or less preserves endothelial vasodilatory function and might therefore protect against atherosclerosis.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2003. 78 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1263
Keyword
Medicine, Atherosclerosis, endothelium, vasodilation, nitric oxide, fatty acid, meal, diet, lipoprotein, cyclooxygenase, diclophenac, L-arginine, LDL, human, Medicin
National Category
Dermatology and Venereal Diseases
Research subject
Internal Medicine
Identifiers
urn:nbn:se:uu:diva-3424 (URN)91-554-5633-2 (ISBN)
Public defence
2003-05-26, Aulan, Thoraxhuset - ingång 50, Akademiska sjukhuset, Uppsala, 09:15
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Available from: 2003-05-06 Created: 2003-05-06 Last updated: 2010-10-21Bibliographically approved

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Steer, PeterKarlström, BritaBerne, ChristianVessby, BengtLind, Lars

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