A statistical experimental design to study factors affecting enantioseparation of propranolol by capillary electrophoresis with cellobiohydrolase (Cel7A) as chiral selector.
2002 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 23, no 14, 2306-2319 p.Article in journal (Refereed) Published
The capillary electrophoretic enantioseparation of rac-propranolol using cellobiohydrolase Tr Cel7A as selector was optimized by an unbiased statistical experimental design. A set of pre-experiments was performed in order to identify critical experimental factors. In the definitive chemometric design pH, ranging from 5 to 7, ionic strength ranging between 0.01 and 0.02 and organic solvent additive in concentration from 1 to 19% v/v were studied. The response surface plot revealed a separation optimum in the pH interval studied. When all parameters were taken into account, a background electrolyte consisting of 0.016 M bistris-acetate buffer with pH 6.5 and 17% v/v acetonitrile gave the optimum separation. The significance of the statistical design was confirmed by the generally good agreement obtained between predicted response and actual experimental data.
Place, publisher, year, edition, pages
2002. Vol. 23, no 14, 2306-2319 p.
Capillary electrophoresis, chiral separation, CBHI, propranolol, statistical experimental design
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-91000ISI: 000177337000021PubMedID: 12210237OAI: oai:DiVA.org:uu-91000DiVA: diva2:163559