Cell adhesion and tissue factor upregulation in oxygenators used during coronary artery bypass grafting are modified by the Corline Heparin Surface
2002 (English)In: Scandinavian Cardiovascular Journal, ISSN 1401-7431, Vol. 36, no 6, 351-357 p.Article in journal (Refereed) Published
OBJECTIVE: Cardiopulmonary bypass (CPB) is associated with inflammatory response and activation of coagulation. We investigated the influence of a new heparin surface on the activation of cells retrieved from oxygenators used during coronary artery bypass grafting (CABG).
DESIGN: Sixty patients undergoing CABG with CPB were randomly assigned to either uncoated or completely Corline Heparin Surface (CHS)-coated circuits with one of three different levels of systemic heparin: standard, high or low. At end of surgery adhered cells were retrieved from the oxygenators and cell count, tissue factor (TF)- and CD11b-expression on monocytes and monocytic TFmRNA were analysed.
RESULTS: The heparin coating of the oxygenator prevented adhesion of granulocytes, monocytes and platelets. TF-expression on monocytes from the oxygenators was significantly higher than on circulating cells in all groups. Monocytes from the uncoated oxygenators showed low levels of TF-expression with high levels of TFmRNA. The coated group with high level of heparin showed higher surface-expression of TF with low levels of TFmRNA.
CONCLUSION: The CHS was most biocompatible with the standard level of heparin used during CABG whereas elevation of systemic heparin rather increased the activation and TF upregulation in monocytes from oxygenators.
Place, publisher, year, edition, pages
2002. Vol. 36, no 6, 351-357 p.
Tissue Factor, Cabg, Heparin, Leukocyte
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-91004DOI: 10.1080/140174302762659076PubMedID: 12626202OAI: oai:DiVA.org:uu-91004DiVA: diva2:163566