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Expression of the vascular endothelial growth factors B and C and their receptors in human endometrium during the menstrual cycle
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Obstetrics and Gynaecology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Obstetrics and Gynaecology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health, Obstetrics and Gynaecology.
2002 (English)In: Acta Obstetricia et Gynecologica Scandinavica, ISSN 0001-6349, E-ISSN 1600-0412, Vol. 81, no 9, 817-824 p.Article in journal (Refereed) Published
Abstract [en]

Background. 

Endometrial angiogenesis is important for bleeding control and fertility, and is believed to be regulated by angiogenic growth factors under the influence of ovarian steroids.

Objective. 

To document the pattern of expression of the two angiogenic growth factors, vascular endothelial growth factor (VEGF)-B and VEGF-C, and of their receptors, VEGFR-1, VEGFR-2, and VEGFR-3, in order to investigate their possible role in the regulation of angiogenesis in normal cycling human endometrium.

Material and methods. 

Cryosections of endometrial biopsy specimens obtained from 17 healthy women before laparoscopic sterilization were examined by immunohistochemistry.

Results. 

Vascular endothelial growth factor and VEGF-C and their receptors were all expressed in and around endometrial blood vessels, with no obvious menstrual cycle-dependent changes in expression except for VEGFR-2, which showed stronger expression during the early secretary phase. Stromal blood vessel density did not change significantly during the menstrual cycle.

Conclusion. 

The presence and pattern of expression of these angiogenic growth factors and their receptors suggest that they participate in the regulation of normal endometrial angiogenesis.

Place, publisher, year, edition, pages
2002. Vol. 81, no 9, 817-824 p.
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:uu:diva-91181DOI: 10.1034/j.1600-0412.2002.810903.xPubMedID: 12225295OAI: oai:DiVA.org:uu-91181DiVA: diva2:163828
Available from: 2003-12-16 Created: 2003-12-16 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Human Endometrial Angiogenesis: An Immunohistochemical Study of the Endometrial Expression of Angiogenic Growth Factors and Their Corresponding Receptors
Open this publication in new window or tab >>Human Endometrial Angiogenesis: An Immunohistochemical Study of the Endometrial Expression of Angiogenic Growth Factors and Their Corresponding Receptors
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The human endometrium undergoes dramatic changes in morphology and function during the menstrual cycle. Recurrent angiogenesis (the formation of new blood vessels) is of utmost importance for oxygen supply and nourishment of the rapidly growing endometrial tissue.

The importance of some growth factors known to stimulate new blood vessel formation both in vivo and in vitro in non-uterine tissues, for endometrial angiogenesis, was studied. Further, the possible relationship between the patterns of expression of some angiogenic growth factors and bleeding disturbances during the use of a progestin-only intrauterine contraceptive device was analyzed. Different ways of determining changes in the endometrial vascular density during the menstrual cycle were also evaluated.

The expression of the angiogenic growth factors vascular endothelial growth factors (VEGF) A, B, C, and D, fibroblast growth factor 2 (FGF-2), and epidermal growth factor (EGF) and their receptors was analyzed using immunohistochemistry.

VEGF-A, -B and -C, FGF-2 and EGF and their receptors were all found to be expressed in normal human endometrium, especially in and/or around blood vessels, supporting the hypothesis that these peptides most probably contribute to the regulation of angiogenesis and blood vessel function in normal human endometrium.

There were differences in expression of some of the studied ligands and receptors in endometrium from users of an LNG-IUS with and without bleeding disturbances. We conclude that changes in the expression of these growth factors and receptors might be involved in the formation of fragile and dysfunctional blood vessels that subsequently give rise to bleeding disturbances.

The three different methods that were applied for calculating endometrial blood vessel density showed similar results and none of them indicated any significant changes during the menstrual cycle. Angiogenesis thus seems to occur mainly by blood vessel elongation and the angiogenic activity is probably related to changes in endometrial thickness and coiling of the spiral arteries.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2004. 50 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1315
Keyword
Medicine, Human endometrium, angiogenesis, menstrual cycle, endothelial cells, immunohistochemistry, western blot, VEGF, VEGFR, FGF-2, FGFR, EGF, EGFR, levonorgestrel-releasing intrauterine system, Medicin
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:uu:diva-3900 (URN)91-554-5831-9 (ISBN)
Public defence
2004-01-30, Rosénsalen, Kvinnokliniken, Akademiska sjukhuset ing 95, Akademiska sjukhuset ing 95, Uppsala, 09:15
Opponent
Supervisors
Available from: 2003-12-16 Created: 2003-12-16Bibliographically approved

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