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The use of inorganic elemental standards in the quantification of proteins and biomolecular compounds by inductively coupled plasma spectrometry
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry, Analytical Chemistry.
2002 In: J. Anal. At. Spectrom, Vol. 17, no 5, 491-496 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2002. Vol. 17, no 5, 491-496 p.
URN: urn:nbn:se:uu:diva-91315OAI: oai:DiVA.org:uu-91315DiVA: diva2:164005
Available from: 2004-01-30 Created: 2004-01-30Bibliographically approved
In thesis
1. Coupled Liquid Separation and Spectrometric Detection of Organic Compounds Containing Hetero-atoms
Open this publication in new window or tab >>Coupled Liquid Separation and Spectrometric Detection of Organic Compounds Containing Hetero-atoms
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis exemplifies the strength in the combination of inductively coupled plasma (ICP) spectrometry and electrospray ionization tandem mass spectrometry ESI-MS/MS as detection techniques for liquid chromatography (LC) in the search for and investigation of compounds that bind or can bind hetero-atoms. Furthermore, some aspects involved in the coupling of LC and ICP spectrometry and quantification without identical standards have been studied.

The importance of using a separation step in combination with ICP spectrometry was shown for urine and blood plasma samples from patients treated with boron neutron capture therapy. In addition to the carrier molecule used in the therapy, one major and a few possible minor metabolites were found in the urine samples. One fragment mass of the major metabolite was obtained with LC-ESI-MS/MS. Liquid chromatography coupled to ICP-MS was also shown to be a valuable tool for fingerprinting metal-binding compounds in complex matrices, such as siderophores (iron-complexing compounds) in soil. The presence of at least two siderophores in a field soil solution sample could be revealed by LC-ICP-MS. Their identities could thereafter be determined by LC-ESI-MS/MS.

The non-UV-absorbing o-carboranylalanine could be quantified in relation to its degradation products by LC-ICP-AES, which provided information about the mechanism behind the degradation. Moreover, LC-ICP spectrometry was shown to provide an accurate quantification of biomolecules (bias < 10 %) when evaluated from external calibration graphs based on inorganic elemental standards.

Finally, the causes of the large decrease in boron signal seen when adding acetonitrile to the LC mobile phase in LC-ICP-MS was investigated in some detail. Space charge effects might explain a large part of the depression from carbon species on the boron signal.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2004. 47 p.
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 933
Analytical chemistry, Analytisk kemi
National Category
Analytical Chemistry
urn:nbn:se:uu:diva-3977 (URN)91-554-5863-7 (ISBN)
Public defence
2004-02-27, B42, BMC, Husargatan 3, Uppsala, 10:15
Available from: 2004-01-30 Created: 2004-01-30 Last updated: 2013-09-24Bibliographically approved

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