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Purification of substance P endopeptidase (SPE) activity in human spinal cord and subsequent comparative studies with SPE in cerebrospinal fluid and with chymotrypsin
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience.
1998 In: Journal of Molecular Recognition, Vol. 11, 266-269 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
1998. Vol. 11, 266-269 p.
Identifiers
URN: urn:nbn:se:uu:diva-91492OAI: oai:DiVA.org:uu-91492DiVA: diva2:164249
Available from: 2004-03-31 Created: 2004-03-31Bibliographically approved
In thesis
1. Substance P Endopeptidase: Purification and Characterizataion of Enzyme Activity and Evaluation of its Function during Stressful Condition
Open this publication in new window or tab >>Substance P Endopeptidase: Purification and Characterizataion of Enzyme Activity and Evaluation of its Function during Stressful Condition
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The purification and biochemical characterization of the substance P (SP) hydrolyzing enzyme, substance P endopeptidase (SPE), have been carried out; with subsequent orientation in neurobiological fundamental processes involved in opioid dependence, withdrawal, and heat-stress.

SPE was purified from rat spinal cord, human spinal cord and cerebrospinal fluid (CSF), rat ventral tegemental area (VTA), and rat hippocampus. The enzyme activity was found to release the biologically active fragments SP(1-7) and SP(1-8) as major products. The purified enzymes were characterized with regard to their biochemical and kinetic properties. The typical SPE is neither inhibited by phosphoramidon nor captopril nor phenylmethanesulfonylflourid (PMSF). In comparison to other known proteases SPE differed in characteristics regarding substrate specificity, inhibition-profile, cleavage pattern, and other kinetic parameters. The technically very delicate approach of micro purification of SPE from the rat ventral tegemental area (VTA) (this is a very small tissue), turned out to be possible with the ÄKTA™-purifier system. Studies revealed a crucial role of SPE in a series of clinically important neuropathological conditions, such as opioid tolerance, and withdrawal (SPE, increased); and heat-stress (SPE, increased). These findings emerged from assessment of enzyme activity in hypothalamus, nucleus accumbens (NAc) periaqueductal gray (PAG), pituitary, striatum, substantia nigra (SN), VTA, spinal cord. Viewing the role of SPE in morphine tolerance, it was possible to note regional differences with a decrease in PAG, and striatum, whereas an increase was seen in SN, and VTA. After heat-stress treatment, SPE was raised in several regions (cerebral cortex, hippocampus, diencephalon, cerebellum, spinal cord), and the most precise observation of this was located to the hippocampus structure.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2004. 56 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1331
Keyword
Pharmacology, Substance P Endopeptidase, Neuropeptide, Central Nervous System, Purification, Drug Dependence, Heat Stress, Farmakologi
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-4130 (URN)91-554-5908-0 (ISBN)
Public defence
2004-04-29, sal B41, BMC, Uppsala, 09:15
Opponent
Supervisors
Available from: 2004-03-31 Created: 2004-03-31Bibliographically approved

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