Quaternary ammonium substituted agarose as surface coating in capillary electrophoresis
2004 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 129, 410-415 p.Article in journal (Refereed) Published
A novel positively charged polymer of quaternary ammonium substituted agarose (Q-agarose) has been synthesized and explored for use as a coating in capillary electrophoresis. The fast and simple coating procedure is based on a multi-site electrostatic interaction between the polycationic agarose polymer and the negatively charged fused-silica surface. By simply flushing fused-silica capillaries with hot polymer solution a positively charged, hydrophilic deactivation layer is achieved. The polymer surface provides an intermediate electroosmotic flow of reversed direction, over a range of pH 2-11, compared to unmodified fused-silica. The coating procedure was highly reproducible with an RSD of 4%, evaluated as the electroosmotic flow mobility for 30 capillaries prepared at 10 different occasions. The application of Q-agarose coated capillaries in separation science was investigated using a set of basic drugs and model proteins and peptides. Due to the intermediate electroosmotic flow generated, the resolution of basic drugs could be increased, compared to using bare fused-silica capillaries. Moreover, the coating enabled separation of proteins and peptides with efficiencies up to 300.000 plates m(-1).
Place, publisher, year, edition, pages
2004. Vol. 129, 410-415 p.
IdentifiersURN: urn:nbn:se:uu:diva-91605DOI: 10.1039/B316492FPubMedID: 15116232OAI: oai:DiVA.org:uu-91605DiVA: diva2:164397