Identification of Adjuvants that Enhance the Therapeutic Antibody Response to Host IgE
2004 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 22, no 21-22, 2873-2880 p.Article in journal (Refereed) Published
In the development of a novel vaccine against atopic allergies, we have screened for adjuvants that enhance the therapeutic antibody response against self immunoglobulin E (IgE). The response against self IgE is induced by administration of a vaccine antigen, which contains both self and non-self IgE regions, together with an adjuvant. We evaluated five commonly used adjuvants; Freund's, aluminium hydroxide, ISCOMs, Montanide ISA 51 and Montanide ISA 720, and found that the mineral oil-based adjuvants; Montanide ISA 51 and Freund's induced at least 5-10-fold higher anti-self IgE titers than any of the other candidates. However, with one exception, Alum, the immune responses against the carrier, i.e. the non-self regions, were similar for all adjuvants, indicating that the ability to induce responses against self and non-self antigens differ among adjuvants. The responses against non-self IgE were more than 50-fold higher than antibody responses against self IgE in both the Freund's and Montanide 51-administered animals, indicating that the response against self molecules is markedly inhibited by tolerance-inducing mechanisms. Co-administration of Montanide ISA 51 with immuno-stimulatory substances from bacteria; muramyldipeptide (MDP), monophosphoryl-lipid A (MPL) or a formyl-methionine-containing tripeptide (fMLP), did not elevate the anti-self IgE response. Hence, adjuvants based on pure mineral oil without additional immuno-stimulatory substances appear to be the best adjuvant candidates in therapeutic vaccines aimed at regulating the in vivo levels of self-proteins.
Place, publisher, year, edition, pages
2004. Vol. 22, no 21-22, 2873-2880 p.
Acetylmuramyl-Alanyl-Isoglutamine/pharmacology, Adjuvants; Immunologic/*pharmacology, Aluminum Hydroxide/pharmacology, Animals, Autoantibodies/analysis/biosynthesis, Autoantigens/immunology, Enzyme-Linked Immunosorbent Assay, Female, Hypersensitivity; Immediate/immunology, Immunization Schedule, Immunization; Secondary, Immunoglobulin E/analysis/*biosynthesis/*immunology, Lipid A/pharmacology, Mineral Oil/pharmacology, Opossums/immunology, Plant Oils/pharmacology, Rats, Rats; Wistar
Engineering and Technology Natural Sciences
IdentifiersURN: urn:nbn:se:uu:diva-91729DOI: 10.1016/j.vaccine.2003.12.029PubMedID: 15246623OAI: oai:DiVA.org:uu-91729DiVA: diva2:164557