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Evaluations of the stability of sheathless electrospray ionization mass spectrometry emitters using electrochemical techniques
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
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2001 In: Analytical Chemistry, ISSN 0003-2700, Vol. 73, no 19, 4607-4616 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2001. Vol. 73, no 19, 4607-4616 p.
Identifiers
URN: urn:nbn:se:uu:diva-91886OAI: oai:DiVA.org:uu-91886DiVA: diva2:164755
Available from: 2004-05-25 Created: 2004-05-25Bibliographically approved
In thesis
1. Development of Sheathless Electrospray Mass Spectrometry and Investigations of Associated Electrochemical Processes – A Fairy Tale
Open this publication in new window or tab >>Development of Sheathless Electrospray Mass Spectrometry and Investigations of Associated Electrochemical Processes – A Fairy Tale
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Utveckling av lågflödeselektrospray-masspektrometri samt undersökningar av associerade elektrokemiska processer – en fésaga
Abstract [en]

In microscale separations, such as capillary electrophoresis and -liquid chromatography, the liquid flow rates are in the order of nanoliters per second. If such flow rates are to be interfaced with a mass spectrometer (MS) using electrospray (ES) ionization, without loss of separation efficiency, each fraction of the analyte zone must remain undisturbed by the high voltage contact necessary for ES. One design that accomplishes this is the pure sheathless approach, where a thin, vapor deposited metal film covers the outside of the electrospray emitter tip.

This thesis describes the development of such sheathless emitters. The lifetimes of polymer embedded gold (“fairy dust”) or graphite (“black dust”) emitters were shown to by far exceed those of previously used conductive films. In addition, the production of emitters with these coatings was substantially simplified. The increase in durability was found to be due to enhanced resistance towards the electrochemical processes associated with ES. In analogy, the reasons for the limited durability of previously used methods were correlated with their tendency to oxidize, or be mechanically removed, during electrochemical reactions.

Electrochemical processes associated with the electrospray potential were also found to seriously disturb analyses in which porous graphitic carbon was used as the separation medium. A proper choice of grounding point locations eliminated these disturbances.

At last, the differences regarding analytical performance of several sheathless interface configurations, used in capillary liquid chromatography, were examined. The best performance was obtained when a pure sheathless emitter with a conductive layer of polyimide and graphite was coupled to the LC column through a Teflon sleeve.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2004. 41 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 989
Keyword
Analytical chemistry, electrospray, sheathless, mass spectrometry, capillary electrophoresis, electrochemistry, chromatography, interface, fairy dust, gold, Analytisk kemi
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-4293 (URN)91-554-5989-7 (ISBN)
Public defence
2004-09-10, Room B 41, BMC - Uppsala Biomedical Center, Husargatan 3, Uppsala, 10:15
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Supervisors
Available from: 2004-05-25 Created: 2004-05-25Bibliographically approved

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