IgE-receptor activation induces survival and Bfl-1 expression in human mast cells but not basophils
2006 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 61, no 9, 1040-1046 p.Article in journal (Refereed) Published
Background: The contribution of mast cells to the pathology of allergic diseases are facilitated by their long life span in tissue and ability to regranulate. Bcl-2 genes are one of the main regulators of cell death and survival. The aim of this study was to elucidate the mechanisms responsible for mast cell survival in allergy.
Methods: Bcl-2 family gene expression in human mast cells and basophils was analyzed by ribonuclease protection assay and by reverse-transcriptase polymerase chain reaction. Cell survival was measured by mixing cells with the vital dye, trypan blue, and the number of living cells was enumerated. Apoptotic cells were measured by a Cell Death Detection ELISA.
Results: We found that cross-linking of Fc epsilon RI on human cord blood cultured mast cells (CBCMCs) promoted cell survival and induced expression of the pro-survival gene Bfl-1. CBCMCs were found to express both Bfl-1 and Bfl-1S, two splicing variants of Bfl-1. Bfl-1 induction was mediated through Syk, PI3-kinase and intracellular calcium mobilization, since piceatannol, wortmannin and EDTA, respectively, significantly reduced Bfl-1 expression levels. In contrast to CBCMCs, no evidence was found for Bfl-1 expression and survival promotion in human basophils.
Conclusions: Immunoglobulin E (IgE)-dependent activation-induced mast cell survival was correlated with Bfl-1 gene upregulation, providing a possible explanation for mast cell longevity in allergic reactions.
Place, publisher, year, edition, pages
2006. Vol. 61, no 9, 1040-1046 p.
A1, basophil, Bcl-2 family members, Bfl-1, Fc epsilon RI, mast cell
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-92471DOI: 10.1111/j.1398-9995.2006.01024.xISI: 000239514400003PubMedID: 16918505OAI: oai:DiVA.org:uu-92471DiVA: diva2:165559