Active smoking and a history of smoking are associated with enhanced prostaglandin F(2alpha), interleukin-6 and F(2)-isoprostane formation in elderly men
2005 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 181, no 1, 201-207 p.Article in journal (Refereed) Published
The underlying mechanisms by which smoking induces cardiovascular diseases are largely unknown. The effect of smoking status on the cyclooxygenase (COX)-mediated inflammatory indicator prostaglandin F(2alpha) (PGF(2alpha)) has never been studied. Associations of cytokines and antioxidants and smoking status, have shown conflicting results. Urinary 15-keto-dihydro-PGF(2alpha) (a major metabolite of PGF(2alpha)), serum interleukin-6 (IL-6) and high sensitivity C-reactive protein (hsCRP), serum amyloid protein A (SAA), urinary 8-iso-PGF(2alpha) (an F(2)-isoprostane, indicator of oxidative stress), and serum alpha-tocopherol were quantified in a population-based sample (n = 642) of 77-year old men without diabetes. Fifty-five men were current smokers and 391 former smokers. Inflammatory indicators were increased in current smokers (15-keto-dihydro-PGF(2alpha), P < 0.001; IL-6, P = 0.01) than non-smokers. 8-iso-PGF(2alpha) was increased (P < 0.01) and alpha-tocopherol reduced (P < 0.001) in current smokers. Further, former smokers had increased formation of 15-keto-dihydro-PGF(2alpha), IL-6 and 8-iso-PGF(2alpha) compared non-smokers. This is the first study to show that smokers have increased PGF(2alpha) formation, thus enhanced COX-mediated inflammation, in addition to elevated levels of cytokines and isoprostanes. Subclinical COX- and cytokine-mediated inflammation and oxidative stress are ongoing processes not only in active smokers but also in former smokers which may contribute to the accelerated atherosclerosis associated with smoking.
Place, publisher, year, edition, pages
2005. Vol. 181, no 1, 201-207 p.
Aged, Aging/*metabolism, Antioxidants/metabolism, Biological Markers/blood/urine, C-Reactive Protein/metabolism, Cohort Studies, Cytokines/metabolism, Dinoprost/analogs & derivatives/*biosynthesis/urine, F2-Isoprostanes/*biosynthesis, Humans, Inflammation/etiology, Interleukin-6/*biosynthesis/blood, Longitudinal Studies, Male, Oxidative Stress, Prostaglandin-Endoperoxide Synthases/metabolism, Smoking/*adverse effects, Smoking Cessation, Time Factors, Tocopherols/blood
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-92656DOI: 10.1016/j.atherosclerosis.2004.11.026PubMedID: 15939073OAI: oai:DiVA.org:uu-92656DiVA: diva2:165817