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Brain Lipid Composition in Postnatal Iron-Induced Motor Behavior Alterations Following Chronic Neuroleptic Administration in Mice
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry, Analytical Chemistry.
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In: Neuroscience LettersArticle in journal (Refereed) Submitted
URN: urn:nbn:se:uu:diva-93098OAI: oai:DiVA.org:uu-93098DiVA: diva2:166472
Available from: 2005-04-29 Created: 2005-04-29 Last updated: 2011-03-21
In thesis
1. Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection: A Targeted Lipidomics Approach
Open this publication in new window or tab >>Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection: A Targeted Lipidomics Approach
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis covers a wide range of analytical method development for lipid analysis in complex biological samples; from sample preparation using pressurized fluid extraction (PFE) and separation with reversed phase capillary liquid chromatography (RP-LC) to detection by electrospray ionization mass spectrometry (ESI/MS) and tandem MS.

The requirements for fast, reliable and selective extraction methods with minimal usage of solvents have accelerated the development of new extraction techniques. PFE is one of the new automated, fast and efficient liquid extraction techniques which use elevated temperature and pressure with standard liquid solvents. In this thesis the reliability and efficiency of the PFE technique was investigated for the extraction of total lipid content from cod, herring muscle and human brain tissue as well as for pesticides from fatty foodstuffs. Improved or comparable efficiencies were achieved with reduced time and solvent consumption as compared to traditional methods.

A RP-LC coupled online to ESI/MS for the analysis of phosphatidylcholine (PC) and sphingomyelin (SM) molecular species was developed and used for the analysis of brain lipids from eight groups of mice treated with vehicle and various neuroleptics. The effect of postnatal iron administration in lipid composition and behavior was investigated. Whether or not these effects could be altered by subchronic administration of the neuroleptics (clozapine and haloperidol) were examined. The results support the hypothesis that an association between psychiatric disorders, behavior abnormalities and lipid membrane constitution in the brain exists.

Finally, a tandem MS precursor ion scan was used to analyze the developmental profile of brain sulfatide accumulation in arylsulfatase A (ASA) deficient (ASA -/-) as compared to wild type control (ASA +/+) mice. The ASA -/- mice were developed as a model of the monogenic disease metachromatic leukodystrophy with an established deficiency of the lysosomal enzyme ASA. The results showed that an alteration in the composition of sulfatide molecular species was observed between the ASA -/- and ASA +/+ mice.

This thesis shows that modern analytical methods can provide new insights in the extraction and analysis of lipids from complex biological samples.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2005. 48 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 56
Analytical chemistry, electrospray ionization, lipids, liquid chromatography, mass spectrometry, pesticides, phosphatidylcholine, pressurized fluid extraction, sphingomyelin, sulfatides, Analytisk kemi
National Category
Analytical Chemistry
urn:nbn:se:uu:diva-5810 (URN)91-554-6259-6 (ISBN)
Public defence
2005-05-20, Room B41, Biomedical Center (BMC), Uppsala, 13:15
Available from: 2005-04-29 Created: 2005-04-29Bibliographically approved

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