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Imaging of peptide adsorption to microfluidic channels in a plastic disc using a positron emitting radionuclide
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Chemistry.
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2005 In: Lab on a chip, Vol. 5, 756-763 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2005. Vol. 5, 756-763 p.
URN: urn:nbn:se:uu:diva-93266OAI: oai:DiVA.org:uu-93266DiVA: diva2:166696
Available from: 2005-09-02 Created: 2005-09-02Bibliographically approved
In thesis
1. Synthesis, Characterisation and Application of 68Ga-labelled Macromolecules
Open this publication in new window or tab >>Synthesis, Characterisation and Application of 68Ga-labelled Macromolecules
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The positron emitting radionuclide 68Ga (T1/2 = 68 min) might become of practical interest for clinical positron emission tomography (PET). The metallic cation, 68Ga(III), is suitable for complexation with chelators, either naked or conjugated with biological macromolecules. Such labelling procedures require pure and concentrated preparations of 68Ga(III), which cannot be sufficiently fulfilled by the presently available 68Ge/68Ga generator eluate. This thesis presents methods to increase the concentration and purity of 68Ga obtained from a commercial 68Ge/68Ga generator. The use of the preconcentrated and purified 68Ga eluate along with microwave heating allowed quantitative 68Ga-labelling of peptide conjugates within 15 min. The specific radioactivity of the radiolabelled peptides was improved considerably compared to previously applied techniques using non-treated generator eluate and conventional heating. A commercial 68Ge/68Ga generator in combination with the method for preconcentration/purification and microwave heated labelling might result in an automated device for 68Ga-based radiopharmaceutical kit production with quantitative incorporation of 68Ga(III).

Macromolecules were labelled with 68Ga(III) either directly or via a chelator. The bifunctional chelator, DOTA, was conjugated in solution to peptides, an antibody and oligonucleotides. The peptides had varied pI values, constitution, and length ranging from 8 to 53 amino acid residues. The oligonucleotides were of various sequences and length with modifications in backbone, sugar moiety and both 3' and 5' ends with a molecular weight up to 9.8 kDa. The bioconjugates were labeled with 68Ga(III), and the resulting tracers were characterised chemically and biologically. The identity of the 68Ga-labelled bioconjugates was verified. The tracers were found to be stable and their biological activity maintained. Specific radioactivity was shown to be an important parameter influencing the feasibility of accurate imaging data quantification.

Furthermore, 68Ga-labelled peptide imaging was shown to be a useful tool to study peptide adsorption to microstructures in a chemical analysis device.

Place, publisher, year, edition, pages
Uppsala: Kemiska institutionen, 2005. 68 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 73
Chemistry, 68Ga, 68Ge/68Ga generator, radiolabelling, microwave heating, specific radioactivity, peptide, protein, antibody, oligonucleotide, positron emission tomography, tumour, Kemi
National Category
Chemical Sciences
urn:nbn:se:uu:diva-5876 (URN)91-554-6295-2 (ISBN)
Public defence
2005-09-23, Room B21, BMC, Husarg. 3, Uppsala, 13:15
Available from: 2005-09-02 Created: 2005-09-02Bibliographically approved

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