The Saccharomyces cerevisiae ORF YNR064c protein has characteristics of an 'orphaned' epoxide hydrolase
2005 (English)In: Biochimica et Biophysica Acta - Proteins and Proteomics, ISSN 1570-9639, E-ISSN 1878-1454, Vol. 1748, 213-221 p.Article in journal (Refereed) Published
The open reading frame YNR064c in Saccharomyces cerevisiae encodes a protein tentatively assigned as similar to a bacterialdehalogenase. In this study we conclude that the YNR064c protein displays characteristics of an epoxide hydrolase belonging to the a/hhydrolasefold family of enzymes. Endogenous expression of the protein in S. cerevisiae was confirmed and a His-tagged variant of theprotein was heterologously expressed in both Escherichia coli and Pichia pastoris for isolation and characterization. The YNR064c proteindisplayed low but reproducible epoxide hydrolase activity with racemic phenanthrene 9,10-oxide and trans- or cis-stilbene oxide.Phylogenetic analysis of related gene products found in various microorganisms suggested that the YNR064c protein is a member of a newsubclass of a/h-hydrolase fold enzymes.
Place, publisher, year, edition, pages
2005. Vol. 1748, 213-221 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-93346DOI: 10.1016/j.bbapap.2005.01.005OAI: oai:DiVA.org:uu-93346DiVA: diva2:166799