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The Effect of Mesenteric Lymphadenectomy and Kupffer Cell Depletion on Bacterial Translocation
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Surgical Sciences.
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2002 In: Journal of Surgical Research, Vol. 102, no 2, 207-214 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2002. Vol. 102, no 2, 207-214 p.
URN: urn:nbn:se:uu:diva-93478OAI: oai:DiVA.org:uu-93478DiVA: diva2:166964
Available from: 2005-09-15 Created: 2005-09-15Bibliographically approved
In thesis
1. Studies of Experimental Bacterial Translocation
Open this publication in new window or tab >>Studies of Experimental Bacterial Translocation
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

One of the main obstacles to maintaining patients with short bowel syndrome on parenteral nutrition, or successfully transplanting these patients with a small bowel graft, is the many severe infections that occur. Evidence is accumulating that translocating bacteria from the patient’s bowel causes a significant part of these infections. In this thesis bacterial translocation is studied in a Thiry-Vella loop of defunctionalised small bowel in the rat.

Bacterial translocation to the mesenteric lymph nodes (MLNs) occurs in almost 100% of the rats after three days. No systemic spread of bacteria is observed unless there is additional immunosupression with depletion of Kupffer cells in the liver. However, blocking the function of α/β T cells does not increase the translocation. Removal of MLNs does not either aggravate bacterial translocation in the Thiry-Vella loop model. Conversely, after small bowel transplantation translocating bacteria spread systemically if the MLNs are removed.

The Thiry-Vella loop should also be a suitable model for the testing of potentially translocation-inhibiting substances. Reinforcement of the intestinal barrier with glutamine or phosphatidylcholine proved insufficient in decreasing bacterial translocation. Even selective bowel decontamination with tobramycin failed to abolish bacterial translocation. Thus, it seems that the driving force for translocation in this model is strong regardless of the relatively small trauma of intestinal defunctionalisation.

Flow cytometric studies of the immune cells in the spleen MLNs showed a decrease in MHC class II positive T cells in the MLNs of the Thiry-Vella loop. Concurrently the number of macrophages increased with time as observed by immunohistochemistry. The fraction of MHC class II negative macrophages increased in the spleens of rats treated with glutamine.

In conclusion, the Thiry-Vella loop model offers possibilities of immunological as well as mechanistic studies on bacterial translocation from small intestine.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2005. 60 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 71
Surgery, Intestinal barrier, short bowel syndrome, small bowel transplantation, mesenteric lymph nodes, gadolinium chloride, T cell inactivation, glutamine, phosphatidylcholine, rat, Thiry-Vella loop, Kirurgi
National Category
urn:nbn:se:uu:diva-5933 (URN)91-554-6339-8 (ISBN)
Public defence
2005-10-07, Rosénsalen, Akademiska Barnsjukhuset, Ing 95 NB, 751 85 Uppsala, 13:15
Available from: 2005-09-15 Created: 2005-09-15 Last updated: 2011-02-18Bibliographically approved

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