T-cell inhibition does not aggravate bacterial translocation from rat small bowel
2006 (English)In: Transplant Immunology, ISSN 0966-3274, E-ISSN 1878-5492, Vol. 16, no 3-4, 208-214 p.Article in journal (Refereed) Published
Background: T-cell mediated immunity has been proposed to have an important function in the defence against translocating microbes from the gastrointestinal tract. After small bowel transplantation massive T-cell immunosuppression is necessary to avoid rejection. As a consequence, infections with intestinal bacteria are the main contributors to mortality in this setting. This could further imply that T cells are important in limiting bacterial translocation. In a model for bacterial translocation from small bowel in the rat we examined the outcome of T-cell inactivation. Methods: The studies were performed in a model of bacterial translocation from a Thiry-Vella loop of small bowel in the rat. The animals were treated with an anti-α/β T-cell receptor monoclonal antibody (R73). Inhibition of T-cell activation was also made using the immunosuppressive drug cyclosporin A. All animals were sacrificed on day 3 postoperatively and translocation to the mesenteric lymph nodes, liver, spleen, lung and blood was evaluated. Results: Treatment with R73 resulted in an almost complete labelling of T cells but did not result in any increased bacterial translocation compared to animals treated with saline. Neither did immunosuppression with cyclosporin A. Conclusions: In the model of bacterial translocation from a defunctionalised loop of small bowel the inhibition of T cells does not increase bacterial translocation to mesenteric lymph nodes or promote the systemic spread of the translocating bacteria. This indicates that T cells do not have any important protective function against translocating microbes from defunctionalised small bowel.
Place, publisher, year, edition, pages
2006. Vol. 16, no 3-4, 208-214 p.
Cyclosporin, Defunctionalised bowel, Mucosal barrier, R73, Small bowel transplantation, Thiry-Vella loop
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-93479DOI: 10.1016/j.trim.2006.09.024ISI: 000243241900010PubMedID: 17138055OAI: oai:DiVA.org:uu-93479DiVA: diva2:166965