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CD64 (Fcγ-receptor I) cell surface expression on maturing neutrophils from preterm and term newborn infants
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
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2005 (English)In: Acta Paediatrica, ISSN 0803-5253, E-ISSN 1651-2227, Vol. 94, no 3, 295-302 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The expression of CD64 (FcgammaRI) is increased from an almost negligible to a marked level on neutrophils in patients with bacterial infections. CD64 expression on neutrophils might therefore be a potential candidate for the diagnosis of bacterial infections in infants.

AIM: This study was performed to monitor changes of neutrophil expression of CD64 during the postpartum period to further evaluate the usefulness of this analysis. The possible influence on the expression of this receptor by other factors was also investigated, including respiratory distress syndrome (RDS) and preterm rupture of the membranes (PROM).

METHODS: Cell surface expression of CD64 on neutrophils from preterm and term newborn infants and healthy adults was analysed by flow cytometry. The expression of the other Fcgamma receptors, CD32 and CD16, and the complement receptors CD11b/CD18 and CD35 was also analysed for comparison.

RESULTS: Neutrophils from preterm newborn infants showed a moderately increased level of CD64 expression that, during their first month of life, was reduced to the level observed on neutrophils from term newborn infants and adults. In contrast, the level of neutrophil expression of CD32 and CD16 was significantly lower in preterm than term newborn infants and adults. Neutrophils from all groups indicated similar levels of CD11b expression, but the expression on neutrophils from newborn infants increased after birth.

CONCLUSION: Our results showed that neutrophil expression of CD64 is moderately increased in preterm newborn infants at birth. It seems not to be influenced by RDS, PROM or other factors related to preterm birth but by bacterial infection.

Place, publisher, year, edition, pages
2005. Vol. 94, no 3, 295-302 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-93548DOI: 10.1080/08035250410023250PubMedID: 16028647OAI: oai:DiVA.org:uu-93548DiVA: diva2:167057
Available from: 2005-10-04 Created: 2005-10-04 Last updated: 2010-11-30Bibliographically approved
In thesis
1. CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
Open this publication in new window or tab >>CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Background. Newborn infants, especially preterm infants, have an increased susceptibility to serious and overwhelming bacterial as well as fungal infections. Symptoms of septicaemia in especially the very preterm neonates are vague and unspecific. No really good biochemical parameter exists today that can confirm or exclude the existence of neonatal septicaemia. The access to such a test in neonates would be most valuable, not only to assure early institution of effective antibiotic therapy when needed, but also to avoid unnecessary use of antibiotics, thereby reducing the risk of further development of antimicrobial resistance.

Aim. To investigate the possible use of the expression of the phagocyte receptor CD64 (FcγRI) on neutrophils for early diagnosis of bacterial infections with special reference to neonatal septicaemia.

Results. Neutrophils from preterm and term newborn infants, older infants, children, and adults examined during the early phase of a bacterial infection showed a significantly higher expression of CD64 compared with non-infected controls (p<0.001). Neutrophils from even extremely preterm infants expressed CD64 to the same extent as did neutrophils from children and adult patients. The expression of CD64 was not affected by the respiratory distress syndrome (RDS) or by such factors as premature rupture of the membranes, gestational age, steroid treatment before delivery, method of delivery, birth weight or postnatal age.

Major surgery in adults (total hip replacement) did not affect the CD64 expression to an extent comparable to that found during bacterial infections. Indirectly CD64 was found to be at least equal to CRP for differentiation between Influenza A infection and bacterial infections in adults.

Conclusion. CD64 was found to be a specific and reliable marker for early detection of bacterial infections in preterm and term newborn infants, as well as after surgery. For differentiation between bacterial and viral infections it is probably at least as effective as CRP.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2005. 56 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 76
Pediatrics, CD64, FcγRI, Neutrophil, Infection, Newborn, Neonate, Pediatrik
National Category
urn:nbn:se:uu:diva-5974 (URN)91-554-6358-4 (ISBN)
Public defence
2005-10-26, Rosensalen, Akademiska Barnsjukhuset, Uppsala, 09:15
Available from: 2005-10-04 Created: 2005-10-04Bibliographically approved

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