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Neutrophil CD64 (FcgammaRI) expression is a specific marker of bacterial infection: A study on the kinetics and the impact of major surgery
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
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2007 (English)In: Scandinavian Journal of Infectious Diseases, ISSN 0036-5548, Vol. 39, no 6-7, 525-535 p.Article in journal (Refereed) Published
Abstract [en]

Neutrophil CD64 expression is a diagnostic marker for the early detection of bacterial infections. The aim was to investigate the kinetics of neutrophil CD64 expression during bacterial infection and the possible impact of surgical trauma. Blood samples were collected daily during 3 d after admission for analysis by flow cytometry of the surface expressions on neutrophils and monocytes of CD64, CD16, CD32, CD11b/CD18 and CD35, and analysis of serum CRP and blood WBC. Serum concentrations of IFNgamma, G-CSF, IL-6 and IL-8 were also analysed in adults. Eight children and 19 adult patients with bacterial infections, 12 patients admitted for hip-arthroplasty because of coxarthrosis and 30 healthy adults were studied. Neutrophil CD64 was increased all 3 d after start of treatment (p<0.0001) in children and adults with bacterial infections. The postoperative increase after surgery was less than the increase seen during bacterial infections (p<0.0001). CRP, G-CSF, IL-6 and IL-8 were raised both in bacterial infections and after surgery. Our results indicate that the expression of CD64 on neutrophils is a specific sign of bacterial infections. Neutrophil expression of CD64, therefore, seems to be a promising tool for the early detection of bacterial infections even during surgery.

Place, publisher, year, edition, pages
2007. Vol. 39, no 6-7, 525-535 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-93549DOI: 10.1080/00365540601113693ISI: 000248339200005PubMedID: 17577814OAI: oai:DiVA.org:uu-93549DiVA: diva2:167058
Available from: 2005-10-04 Created: 2005-10-04 Last updated: 2011-01-24Bibliographically approved
In thesis
1. CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
Open this publication in new window or tab >>CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Background. Newborn infants, especially preterm infants, have an increased susceptibility to serious and overwhelming bacterial as well as fungal infections. Symptoms of septicaemia in especially the very preterm neonates are vague and unspecific. No really good biochemical parameter exists today that can confirm or exclude the existence of neonatal septicaemia. The access to such a test in neonates would be most valuable, not only to assure early institution of effective antibiotic therapy when needed, but also to avoid unnecessary use of antibiotics, thereby reducing the risk of further development of antimicrobial resistance.

Aim. To investigate the possible use of the expression of the phagocyte receptor CD64 (FcγRI) on neutrophils for early diagnosis of bacterial infections with special reference to neonatal septicaemia.

Results. Neutrophils from preterm and term newborn infants, older infants, children, and adults examined during the early phase of a bacterial infection showed a significantly higher expression of CD64 compared with non-infected controls (p<0.001). Neutrophils from even extremely preterm infants expressed CD64 to the same extent as did neutrophils from children and adult patients. The expression of CD64 was not affected by the respiratory distress syndrome (RDS) or by such factors as premature rupture of the membranes, gestational age, steroid treatment before delivery, method of delivery, birth weight or postnatal age.

Major surgery in adults (total hip replacement) did not affect the CD64 expression to an extent comparable to that found during bacterial infections. Indirectly CD64 was found to be at least equal to CRP for differentiation between Influenza A infection and bacterial infections in adults.

Conclusion. CD64 was found to be a specific and reliable marker for early detection of bacterial infections in preterm and term newborn infants, as well as after surgery. For differentiation between bacterial and viral infections it is probably at least as effective as CRP.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2005. 56 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 76
Keyword
Pediatrics, CD64, FcγRI, Neutrophil, Infection, Newborn, Neonate, Pediatrik
National Category
Pediatrics
Identifiers
urn:nbn:se:uu:diva-5974 (URN)91-554-6358-4 (ISBN)
Public defence
2005-10-26, Rosensalen, Akademiska Barnsjukhuset, Uppsala, 09:15
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Available from: 2005-10-04 Created: 2005-10-04Bibliographically approved

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