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Cell surface expression of FcgammaRI (CD64) on neutrophils and monocytes in patients with influenza A, with and without complications
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Friman, infektion)
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2005 (English)In: Scandinavian Journal of Infectious Diseases, ISSN 0036-5548, Vol. 37, no 11-12, 882-889 p.Article in journal (Refereed) Published
Abstract [en]

The expression of the Fcgamma-receptor I (FcgammaRI), CD64 on normal neutrophils is up-regulated during bacterial infections. CD64 is a promising diagnostic tool in the diagnosis of acute infections. The aim was to study surface expressions of CD64 on neutrophils and monocytes in patients with influenza A with and without complications and evaluate these as diagnostic tools in comparison with serum levels of HNL (human neutrophil lipocalin). CD64 expression on neutrophils and monocytes was evaluated by flow cytometry. HNL was assayed by a specific radioimmunoassay. 22 patients with influenza A with or without complications were included and the results compared with those of 29 patients with acute bacterial infections and 29 healthy subjects. Neutrophil expression of CD64 was increased in influenza A with raised proportion expressing CD64 in complicated compared to uncomplicated influenza. The expression was significantly higher in bacterial infections compared to both influenza groups. Serum levels of HNL were raised in all infection groups, but significantly more so in the group with bacterial infection. ROC-curve analysis showed that neutrophil expression of CD64 and the serum levels of HNL had similar diagnostic power in the discrimination between acute bacterial infections and influenza A. Monocyte expression of CD64 was raised in all infections with no differences between subgroups. We conclude that neutrophil expression of CD64 and serum levels of HNL are both promising assays in the distinction between infections caused by bacteria or influenza A, whereas CD64 could identify patients with complications of their influenza A infection.

Place, publisher, year, edition, pages
2005. Vol. 37, no 11-12, 882-889 p.
Keyword [en]
Acute-Phase Proteins, Adult, Aged, Aged; 80 and over, Bacterial Infections/complications/diagnosis/immunology, C-Reactive Protein/metabolism, Case-Control Studies, Humans, Influenza A virus, Influenza; Human/complications/diagnosis/*immunology, Middle Aged, Monocytes/immunology, Neutrophils/immunology, Pneumonia/complications/immunology, Proto-Oncogene Proteins/blood, Receptors; IgG/*metabolism
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-93550DOI: 10.1080/00365540500348929PubMedID: 16308225OAI: oai:DiVA.org:uu-93550DiVA: diva2:167059
Available from: 2005-10-04 Created: 2005-10-04 Last updated: 2010-11-29Bibliographically approved
In thesis
1. CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
Open this publication in new window or tab >>CD64 (FcγRI) Expression on Neutrophil Granulocytes: A Diagnostic Marker of Acute Bacterial Infections
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Background. Newborn infants, especially preterm infants, have an increased susceptibility to serious and overwhelming bacterial as well as fungal infections. Symptoms of septicaemia in especially the very preterm neonates are vague and unspecific. No really good biochemical parameter exists today that can confirm or exclude the existence of neonatal septicaemia. The access to such a test in neonates would be most valuable, not only to assure early institution of effective antibiotic therapy when needed, but also to avoid unnecessary use of antibiotics, thereby reducing the risk of further development of antimicrobial resistance.

Aim. To investigate the possible use of the expression of the phagocyte receptor CD64 (FcγRI) on neutrophils for early diagnosis of bacterial infections with special reference to neonatal septicaemia.

Results. Neutrophils from preterm and term newborn infants, older infants, children, and adults examined during the early phase of a bacterial infection showed a significantly higher expression of CD64 compared with non-infected controls (p<0.001). Neutrophils from even extremely preterm infants expressed CD64 to the same extent as did neutrophils from children and adult patients. The expression of CD64 was not affected by the respiratory distress syndrome (RDS) or by such factors as premature rupture of the membranes, gestational age, steroid treatment before delivery, method of delivery, birth weight or postnatal age.

Major surgery in adults (total hip replacement) did not affect the CD64 expression to an extent comparable to that found during bacterial infections. Indirectly CD64 was found to be at least equal to CRP for differentiation between Influenza A infection and bacterial infections in adults.

Conclusion. CD64 was found to be a specific and reliable marker for early detection of bacterial infections in preterm and term newborn infants, as well as after surgery. For differentiation between bacterial and viral infections it is probably at least as effective as CRP.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2005. 56 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 76
Keyword
Pediatrics, CD64, FcγRI, Neutrophil, Infection, Newborn, Neonate, Pediatrik
National Category
Pediatrics
Identifiers
urn:nbn:se:uu:diva-5974 (URN)91-554-6358-4 (ISBN)
Public defence
2005-10-26, Rosensalen, Akademiska Barnsjukhuset, Uppsala, 09:15
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Available from: 2005-10-04 Created: 2005-10-04Bibliographically approved

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Fjaertoft, GustavPauksens, KarlisVenge, Per

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