Cadmium-induced gene expression changes in the mouse embryo, and the influence of pretreatment with zinc
2006 (English)In: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 22, no 4, 636-646 p.Article in journal (Refereed) Published
Cadmium (Cd) administered to female C57BL/6 mice on gestation day 8 induces a high incidence of anterior neural tube defects (exencephaly). This adverse effect can be attenuated by maternal pretreatment with zinc (Zn). In this study we used replicated microarray analysis and real-time PCR to investigate gene expression changes induced in the embryo 5 and 10h after maternal Cd exposure in the absence or presence of Zn pretreatment. We report nine genes with a transcriptional response induced by Cd, none of which was influenced by Zn pretreatment, and two genes induced only by combined matemal Cd exposure and Zn pretreatment. We discuss the results in relation to the possibility that Cd is largely excluded from the embryo, that the teratogenic effects of Cd may be secondary to toxicity in extraembryonic tissues, and that the primary protective role of Zn may not be to reverse Cd-induced transcription in the embryo.
Place, publisher, year, edition, pages
2006. Vol. 22, no 4, 636-646 p.
cadmium, exencephaly, microarray, mouse embryo, neural tube defects, toxicogenomics, zinc
IdentifiersURN: urn:nbn:se:uu:diva-93851DOI: 10.1016/j.reprotox.2006.05.007ISI: 000241622800014PubMedID: 16842966OAI: oai:DiVA.org:uu-93851DiVA: diva2:167467