In 1982 a Swedish study estimated that 7% of the population had been infected by Toxocara spp and it’s a growing issue due to anthelmintic resistance. The purpose of this project was to study the infective L3 larva stage of Toxocara spp and its interaction with human colorectal adenocarcinoma cells to observe how it affects the gastrointestinal early chemokine secretion. The chemokines CCL20, CXCL1, and IL-8 contribute to the recruitment of neutrophilic granulocytes, lymphocytes, and the inflammatory innate immune response. The primer pairs of the above chemokines and caco2 cells were received from Uppsala university. The Toxocara parasite eggs were purified from dog feces, embryonated in 28 until the L3 stage, formalin treated, and then mechanically hatched. The L3 larva was used in two individual challenge experiments to study the changes in the expression levels of CCL20, CXCL1, and IL-8 in caco2 cells at six hours and 24 hours of exposure. A potential down-regulation of CCL20 in the six hours challenge experiment and a down-regulation of CXCL1 and IL-8 in the 24 hours challenge experiment were identified. This indicates that infection with Toxocara canis L3 larva impacts the early human gastrointestinal immune response, but additional studies are required to confirm this observation.