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Quartz crystal microbalance sensor design: I. Experimental study of sensor response and performance
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
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2007 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, Vol. 123, no 1, 27-34 p.Article in journal (Refereed) Published
Abstract [en]

This paper investigates a novel quartz crystal microbalance (QCM) biosensor with a small and rectangular flow cell along with a correspondingly shaped crystal electrode. The sensor was evaluated with impedance analysis and compared to standard circular sensor crystals and sensor crystals with small circular electrodes. Comparative QCM measurements on an antibody–antigen interaction system were carried out on the rectangular and standard circular sensor systems. Impedance analysis and subsequent data extraction of the three different sensor crystals showed that the smaller sensors had significantly higher Q-values in air, but that liquid load on the electrodes lowered the Q-values radically for all crystals. Under liquid load, Q-values for the standard circular and the rectangular sensors were similar whereas the Q-value for the small circular sensor was 50% higher. QCM experiments showed that the QCM system with rectangular crystal electrodes was fully functional in a liquid environment. The rectangular system showed higher and more rapid responses for series of antibody injections, albeit at a higher noise level than the standard system. The study elucidates a significant potential for improvement of sensor performance by optimising the sensor electrode size and shape together with the flow cell geometry.

Place, publisher, year, edition, pages
2007. Vol. 123, no 1, 27-34 p.
Keyword [en]
QCM, Biosensor, Sensitivity, Mass transport, Protein interactions
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-94271DOI: 10.1016/j.snb.2006.07.027ISI: 000246171200006OAI: oai:DiVA.org:uu-94271DiVA: diva2:168062
Available from: 2006-04-07 Created: 2006-04-07 Last updated: 2011-02-02Bibliographically approved
In thesis
1. Development of Electroacoustic Sensors for Biomolecular Interaction Analysis
Open this publication in new window or tab >>Development of Electroacoustic Sensors for Biomolecular Interaction Analysis
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Biomolecular interaction analysis to determine the kinetics and affinity between interacting partners is important for the fundamental understanding of biology, as well as for the development of new pharmaceutical substances. A quartz crystal microbalance instrument suitable for kinetics and affinity analyses of interaction events was developed. The functionality of the sensor system was demonstrated by development of an assay for relative affinity determination of lectin-carbohydrate interactions.

Sensor surfaces allowing for effective immobilization of one interacting partner is a key functionality of a biosensor. Here, three different surfaces and immobilization methods were studied. First, optimized preparation conditions for sensor surfaces based on carboxyl-terminated self assembled monolayers were developed and were demonstrated to provide highly functional biosensor surfaces with low non-specific binding. Second, a method allowing for immobilization of very acidic biomolecules based on the use of an electric field was developed and evaluated. The electric field made it possible to immobilize the highly acidic C-peptide on a carboxylated surface. Third, a method for antibody immobilization on a carboxyl surface was optimized and the influence of immobilization pH on the immobilization level and antigen binding capacity was thoroughly assessed. The method showed high reproducibility for a set of antibodies and allowed for antibody immobilization also at low pH.

Three broadly different strategies to increase the sensitivity of electroacoustic sensors were explored. A QCM sensor with small resonator electrodes and reduced flow cell dimensions was demonstrated to improve the mass transport rate to the sensor surface. The use of polymers on QCM sensor surfaces to enhance the sensor response was shown to increase the response of an antibody-antigen model system more than ten-fold. Moreover, the application of high frequency thin film bulk acoustic resonators for biosensing was evaluated with respect to sensing range from the surface. The linear detection range of the thin film resonator was determined to be more than sufficient for biosensor applications involving, for instance, antibody-antigen interactions. Finally, a setup for combined frequency and resistance measurements was developed and was found to provide time resolved data suitable for kinetics determination.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 68 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 658
Keyword
biosensor, protein interactions, kinetics, affinity, QCM, quartz crystal microbalance, piezoelectric resonators, dissipation, motional resistance
National Category
Other Industrial Biotechnology Analytical Chemistry
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
urn:nbn:se:uu:diva-107211 (URN)978-91-554-7572-7 (ISBN)
Public defence
2009-09-11, Å80101, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:30 (English)
Opponent
Supervisors
Projects
wisenet
Available from: 2009-08-20 Created: 2009-07-29 Last updated: 2014-11-25Bibliographically approved
2. Microfluidic Devices for Manipulation and Detection of Beads and Biomolecules
Open this publication in new window or tab >>Microfluidic Devices for Manipulation and Detection of Beads and Biomolecules
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis summarises work towards a Lab-on-Chip (LOC). The request for faster and more efficient chemical and biological analysis is the motivation behind the development of the LOC-concept.

Microfluidic devices tend to become increasingly complex in order to include, e.g. sample delivery, manipulation, and detection, in one chip. The urge for smart and simple design of robust and low-cost microdevices is addressed and discussed.

Design, fabrication and characterization of such microdevices have been demonstrated using low-cost polymer and glass microfabrication methods. The manufacturing is feasible, to a large extent, to perform outside the clean-room, and has subsequently been the chosen technique for most of the work. Issues of bonding reliability are solved by using polymer adhesive tapes.

A planar electrocapture device with LOC-compatibility is demonstrated where beads are immobilised and released in a flowing stream. Retention of nanoparticles by means of electric field-flow fractionation using transparent indium tin oxide electrodes is presented. Moreover, a cast PDMS 4-way crossing is enabling a combination of liquid chromatography and capillary electrophoresis to enhance separation efficiency. Sample transport issues and a new flow-cell design in a quartz crystal microbalance bioanalyzer are also investigated. Fast bacteria counting by impedance measurements, much requested by the pharmaceutical industry for biomass monitoring, is carried out successfully.

In conclusion, knowledge in micro system technology to build microdevices have been utilised to manipulate and characterise beads and cells, taking one step further towards viable Lab-on-Chip instruments.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 43 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 166
Keyword
Engineering physics, sensor, micro, dielectrophoresis, impedance, electrode, beads, cell, flow, bacteria, microfabrication, biosensor, electrocapture, microfluidic, planar, Lab on chip, µTAS, micro total analysis system, QCM, dispersion, field-flow fractionation, ITO, design, fabrication, Teknisk fysik
National Category
Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-6746 (URN)91-554-6523-4 (ISBN)
Public defence
2006-04-28, Siegbahnsalen, Ångströmlaboratoriet, Lägerhyddsvägen1, Uppsala, 10:00 (English)
Opponent
Supervisors
Available from: 2006-04-07 Created: 2006-04-07 Last updated: 2011-12-09Bibliographically approved

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Anderson, HenrikVestling, Lars

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