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Design and fabrication of a miniaturised Electrical Field-Flow Fractionation instrument with ITO electrodes
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Technology, Department of Engineering Sciences.
Manuscript (Other academic)
URN: urn:nbn:se:uu:diva-94273OAI: oai:DiVA.org:uu-94273DiVA: diva2:168064
Available from: 2006-04-07 Created: 2006-04-07 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Microfluidic Devices for Manipulation and Detection of Beads and Biomolecules
Open this publication in new window or tab >>Microfluidic Devices for Manipulation and Detection of Beads and Biomolecules
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis summarises work towards a Lab-on-Chip (LOC). The request for faster and more efficient chemical and biological analysis is the motivation behind the development of the LOC-concept.

Microfluidic devices tend to become increasingly complex in order to include, e.g. sample delivery, manipulation, and detection, in one chip. The urge for smart and simple design of robust and low-cost microdevices is addressed and discussed.

Design, fabrication and characterization of such microdevices have been demonstrated using low-cost polymer and glass microfabrication methods. The manufacturing is feasible, to a large extent, to perform outside the clean-room, and has subsequently been the chosen technique for most of the work. Issues of bonding reliability are solved by using polymer adhesive tapes.

A planar electrocapture device with LOC-compatibility is demonstrated where beads are immobilised and released in a flowing stream. Retention of nanoparticles by means of electric field-flow fractionation using transparent indium tin oxide electrodes is presented. Moreover, a cast PDMS 4-way crossing is enabling a combination of liquid chromatography and capillary electrophoresis to enhance separation efficiency. Sample transport issues and a new flow-cell design in a quartz crystal microbalance bioanalyzer are also investigated. Fast bacteria counting by impedance measurements, much requested by the pharmaceutical industry for biomass monitoring, is carried out successfully.

In conclusion, knowledge in micro system technology to build microdevices have been utilised to manipulate and characterise beads and cells, taking one step further towards viable Lab-on-Chip instruments.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 43 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 166
Engineering physics, sensor, micro, dielectrophoresis, impedance, electrode, beads, cell, flow, bacteria, microfabrication, biosensor, electrocapture, microfluidic, planar, Lab on chip, µTAS, micro total analysis system, QCM, dispersion, field-flow fractionation, ITO, design, fabrication, Teknisk fysik
National Category
Engineering and Technology
urn:nbn:se:uu:diva-6746 (URN)91-554-6523-4 (ISBN)
Public defence
2006-04-28, Siegbahnsalen, Ångströmlaboratoriet, Lägerhyddsvägen1, Uppsala, 10:00 (English)
Available from: 2006-04-07 Created: 2006-04-07 Last updated: 2011-12-09Bibliographically approved

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