uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
On the cell cycle of Escherichia coli and cell division in Haloferax mediterranei
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Escherichia coli has served as a model organism for prokaryotes for several decades and is perhaps the most well characterised organism on earth. The use of fluorescence microscopy during recent years has contributed substantially to our understanding of the essential cell cycle processes chromosome replication, chromosome segregation and cell division.

In this thesis, the co-ordination between chromosome replication and cell division has been investigated. It was found that cell division does not have to take place within a certain time after chromosome replication and that in the absence of chromosome replication, cell division is initiated but is most often not completed. Furthermore, the condensation state of non-replicating chromosomes was demonstrated to be severely affected in vivo in the absence of the protein MukB. Chromosome segregation disturbances were quantified in cells with a defective Min system, which is normally involved in directing cell division to the center of the cell. Possible causes for this disturbed chromosome segregation were investigated. Some E. coli strains form branched cells with a high frequency and the mechanism for the formation of branches was investigated. Branches were formed also after inhibition of cell division, showing that the cell division machinery is not required for the emergence of branches.

Prokaryotes are divided into two main, evolutionary defined groups; Archaea and Bacteria, the last common ancestor of which might have lived 3-4 billion years ago. Here, cell division in the archaeon Haloferax mediterranei was demonstrated to share features with cell division in other archaea and bacteria, including E. coli.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis , 2001. , 63 p.
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1104-232X ; 640
Keyword [en]
Cell and molecular biology, Branch, division, Escherichia coli, Haloferax mediterranei, replication, segregation
Keyword [sv]
Cell- och molekylärbiologi
National Category
Biochemistry and Molecular Biology
Research subject
URN: urn:nbn:se:uu:diva-686ISBN: 91-554-5056-3OAI: oai:DiVA.org:uu-686DiVA: diva2:168330
Public defence
2001-05-30, B42 BMC, Uppsala, 10:00
Available from: 2001-05-09 Created: 2001-05-09Bibliographically approved

Open Access in DiVA

No full text
Buy this publication >>

By organisation
Department of Cell and Molecular Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 77 hits
ReferencesLink to record
Permanent link

Direct link