Acid hydrolysis of bacterial cellulose reveals different modes of synergistic action between cellobiohydrolase I and endoglucanase I
1999 (English)In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 266, no 2, 327-334 p.Article in journal (Refereed) Published
Intact and partially acid hydrolyzed cellulose from Acetobacter xylinum were used as model substrates for cellulose hydrolysis by 1,4-β-d-glucan-cellobiohydrolase I (CBH I) and 1,4-β-d-endoglucanase I (EG I) from Trichoderma reesei. A high synergy between CBH I and EG I in simultaneous action was observed with intact bacterial cellulose (BC), but this synergistic effect was rapidly reduced by acid pretreatment of the cellulose. Moreover, a distinct synergistic effect was observed upon sequential endo–exo action on BC, but not on bacterial microcrystalline cellulose (BMCC). A mechanism for endo–exo synergism on crystalline cellulose is proposed where the simultaneous action of the enzymes counteract the decrease of activity caused by undesirable changes in the cellulose surface microstructure.
Place, publisher, year, edition, pages
1999. Vol. 266, no 2, 327-334 p.
cellulase, cellulose, hydrolysis, synergism, simulation
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-94511DOI: 10.1046/j.1432-1327.1999.00853.xISI: 000084444000002OAI: oai:DiVA.org:uu-94511DiVA: diva2:168383