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Polarized protein membrane for high cell seeding efficiency
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Materials Chemistry.
2007 (English)In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, ISSN 1552-4973, Vol. 83, no 2, 472-480 p.Article in journal (Refereed) Published
Abstract [en]

A new type of scaffold for tissue engineering was developed to give enhanced cell seeding in three dimensions. A gradient of either collagen or fibrin protein was prepared, supported by a knitted poly(ethylene terephtalate) PET fabric. The membranes were, after hydrolysis and acetic acid wash, submerged in a protein solution for adsorption followed by immersion into a gelling agent. The immediate contact between the protein solution held by the fabric and the gelling agent resulted in a dense, fibrous protein network with pore sizes around 0.5 μm at the surface, and larger pores of 10-50 μm size throughout the interior of the fabric as observed by scanning electron microscopy. By separating the fabric double layers holding this network, a gradient porosity membrane was produced. To evaluate the fractions of cells trapped in the matrix upon seeding, i.e. the seeding efficiency, 500 μl 3T3 fibroblasts cell suspension containing one million cells was seeded by filtering through the gradient protein membrane. For both the collagen and fibrin membranes, the seeding efficiency was ∼93%, which was significantly higher than that of 28% from the corresponding PET fabric without protein immobilization. Attempt to seed cells from the dense side of the protein networks resulted in no cell penetration into the scaffold. Histology on subsequent culture of the cells in the scaffold demonstrated viability and proliferation in three dimensions throughout the scaffold. This new and simple way of producing scaffolds play an important role when the cells are precious or scarce and cell seeding in three dimensions is important.

Place, publisher, year, edition, pages
2007. Vol. 83, no 2, 472-480 p.
Keyword [en]
Cell adhesion, Membrane, Protein adsorption, Scaffold
National Category
Biological Sciences Chemical Sciences
URN: urn:nbn:se:uu:diva-94593DOI: 10.1002/jbm.b.30819ISI: 000250425400024PubMedID: 17443668OAI: oai:DiVA.org:uu-94593DiVA: diva2:168489
Available from: 2006-05-17 Created: 2006-05-17 Last updated: 2011-01-20Bibliographically approved
In thesis
1. Tailoring of Biomaterials using Ionic Interactions: Synthesis, Characterization and Application
Open this publication in new window or tab >>Tailoring of Biomaterials using Ionic Interactions: Synthesis, Characterization and Application
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The interactions between polymers and components of biological systems are an important area of interest within the fields of tissue engineering, polymer chemistry, medicine and biomaterials. In order to create such a biomimetic material, it must show the inherent ability to reproduce or elicit a biological function. How do we design synthetic materials in order to direct their interactions with biological systems?

This thesis contributes to this research with aspects of how polymers interact with biological materials with the help of ionic interactions. Polyesters, biodegradable or not, may after a hydrolytic cleavage interact ionically with protonated amines by the liberated carboxylate functions. Amines are found in proteins and this fact will help us to anchor proteins to polyester surfaces. Another type of interaction is to culture cells in polymeric materials, i.e. scaffolds. We have been working on compliant substrates, knitted structures, to allow cell culture in three dimensions. A problem that arises here is how to get a high cell seeding efficiency? By working on the interactions between polymers, proteins and finally cells, it is possible to create a polarized protein membrane that allows for very efficient cell seeding, and subsequent three dimensional cell cultures. Finally a synthetic route to taylor interaction was developed. Here a group of polymers known as ionomers were synthesized. In our case ionic end groups have been placed onto biodegradable polycarbonates, we have created amphiphilic telechelic ionomers. Functionalization, anionic or cationic, changes the properties of the material in many ways due to aggregation and surface enrichment of ionic groups. It is possible to add functional groups for a variety of different interactions, for example introducing ionic groups that interact and bind to the complementary charge of proteins or on the other hand one can chose groups to prevent protein interactions, like the phosphorylcholine zwitterionomers. Such interactions can be utilized to modulate the release of proteins from these materials when used in protein delivery applications. The swelling properties, Tg, degradation rate and mechanical properties are among other things that will easily be altered with the choice of functional groups or backbone polymer.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 92 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 193
Chemistry, biodegradable polymers, ionomer, water uptake, protein delivery, protein adsorption, protein membrane, cell seeding efficiency, amphiphillic, inner structure, polarized membrane, Kemi
urn:nbn:se:uu:diva-6924 (URN)91-554-6585-4 (ISBN)
Public defence
2006-06-07, Polhemsalen, Ångströmlaboratoriet, Regementsvägen 1, Uppsala, 10:00
Available from: 2006-05-17 Created: 2006-05-17 Last updated: 2013-09-26Bibliographically approved

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