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3-O sulfated oligosaccharide structures are recognized by anti-heparan sulfate antibody HS4C3
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2006 (English)In: Journal of Biological Chemistry, Vol. 281, 4654-62 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2006. Vol. 281, 4654-62 p.
URN: urn:nbn:se:uu:diva-94738OAI: oai:DiVA.org:uu-94738DiVA: diva2:168702
Available from: 2006-09-08 Created: 2006-09-08 Last updated: 2011-06-28Bibliographically approved
In thesis
1. Design of Oligosaccharide Libraries to Characterize Heparan Sulfate – Protein Interactions
Open this publication in new window or tab >>Design of Oligosaccharide Libraries to Characterize Heparan Sulfate – Protein Interactions
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Heparan sulfates (HSs) are a class of anionic carbohydrate chains found at cell surfaces and in the extracellular matrix where they interact with a number of proteins. HS is characterized by extreme structural heterogeneity, and has been implicated in a number of biological phenomenon like embryogenesis, morphogen gradient formation and signalling of growth factors such as FGF, PDGF etc. Despite the characteristic structural heterogeneity, evidence from compositional studies show that the HS structure is expressed in a tightly regulated manner, implying a functional significance, which is most likely in the modulation of cell behaviour through HS-protein interactions. The lack of molecular tools has, however, hampered the understanding of HS structures with functional significance. This work therefore aims at characterizing the structural requirements on HS involved in the interaction with the anti-HS phage display antibodies HS4C3, AO4B08 and HS4E4 and a selected growth factor PDGF-BB. The characterization was done with the help of tailored oligosaccharide libraries generated from sources bearing structural resemblance to HS.

The work has thus made available tools that preferentially recognize certain structural features on the HS chain and will aid in the further study of HS structure and its regulation. Evidence is also provided to support the notion that HS protein interactions can occur in multiple manners, utilizing any of the structural features on the HS chain.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 38 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 165
Biochemistry, heparan sulfate, oligosaccharide, phage display antibodies, Biokemi
urn:nbn:se:uu:diva-7095 (URN)91-554-6626-5 (ISBN)
Public defence
2006-09-28, C10:301, BMC, Husargatan,3, Uppsala, 09:15
Available from: 2006-09-08 Created: 2006-09-08Bibliographically approved

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Spillmann, Dorothe
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