uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
On-Line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol-Gel Columns
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
Show others and affiliations
2006 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 842, no 1, 70-74 p.Article in journal (Refereed) Published
Abstract [en]

Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol-gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flow electrospray. The importance of desalting this type of sample is demonstrated by an experiment that shows that the signal intensity of a test solution with neurotensin, sprayed directly into the mass spectrometer, decreased from 4.5 x 10(4) Cps to no detectible signal when just 10% urine is added to the sample solution. We suggest that this procedure may find general application for desalting biological samples prior to mass spectrometric analysis.

Place, publisher, year, edition, pages
2006. Vol. 842, no 1, 70-74 p.
Keyword [en]
desalting, on-line, preconcentration, biological sample, mass spectrometry, electrophoresis, octadecyltrimethoxysilane, sol-gel
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-94821DOI: 10.1016/j.jchromb.2006.05.012ISI: 000240712700011PubMedID: 16814621OAI: oai:DiVA.org:uu-94821DiVA: diva2:168812
Available from: 2006-09-15 Created: 2006-09-15 Last updated: 2011-06-14Bibliographically approved
In thesis
1. Column Development in Capillary Electrophoresis and Electrochromatography for Bioanalytical Applications
Open this publication in new window or tab >>Column Development in Capillary Electrophoresis and Electrochromatography for Bioanalytical Applications
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Analysis of biological samples can be a difficult task. This thesis covers a broad aspect of the analytical areas of capillary electrophoresis (CE) and capillary electrochromatography (CEC) in combination with mass spectrometry (MS) that are of great importance for achieving fast, accurate and sensitive bioanalyses. A significantly time reduced and automated system for sample cleanup was developed to greatly simplify the pretreatment process of biological samples with a complex matrix. Desalting and preconcentration of species in urine was conducted and the limit of detection for the antidepressant escitalopram was lowered 10 times. This extraction devise was also successfully incorporated in a chip based platform for the possibility to be a part of multidimensional separation systems. The reduced risk of sample loss leads to improved detection limits, which are usually one the most challenging parts when working with bioanalyses.

In the area of separation, a monomer surface with tailored hydrophobicity was developed to achieve rapid, high efficient separations of complex mixtures. Within five minutes a tryptic digest of a protein could be separated and then identified by a Mascot search.

The applications addressed have been focused on medical conditions which are of highest interest for both physicians and patients. A high throughput analysis of the kynurenine metabolites with CE-MS offers a new method to rapidly examine samples from patients with neurological disorders.

A screening study of possible biomarkers for the two different types of appendicitis, gangraenous and phlegmonous was conducted. Indicative patterns were found for both pre and post surgery of the two types of inflammation as well as between them. The divergences were traced back to the MS peaks obtained in the CE- and CEC-MS setups as possible biomarkers for the two forms of appendicitis. A preliminary study of polycystic ovary syndrome also offered some valuable results for future biomarker identification.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 54 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 216
Analytical chemistry, Capillary electrophoresis (CE), Capillary electrochromatography (CEC), Mass spectrometry (MS), Bioanalysis, Sample pretreatment, Sol-gel, Analytisk kemi
National Category
Chemical Sciences
urn:nbn:se:uu:diva-7124 (URN)91-554-6644-3 (ISBN)
Public defence
2006-10-06, B22, BMC, Uppsala, 10:15 (English)
Available from: 2006-09-15 Created: 2006-09-15 Last updated: 2012-02-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Johannesson, NinaBergquist, Jonas
By organisation
Analytical Chemistry
In the same journal
Journal of chromatography. B
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 409 hits
ReferencesLink to record
Permanent link

Direct link