On-Line Biological Sample Cleanup for Electrospray Mass Spectrometry Using Sol-Gel Columns
2006 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 842, no 1, 70-74 p.Article in journal (Refereed) Published
Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol-gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flow electrospray. The importance of desalting this type of sample is demonstrated by an experiment that shows that the signal intensity of a test solution with neurotensin, sprayed directly into the mass spectrometer, decreased from 4.5 x 10(4) Cps to no detectible signal when just 10% urine is added to the sample solution. We suggest that this procedure may find general application for desalting biological samples prior to mass spectrometric analysis.
Place, publisher, year, edition, pages
2006. Vol. 842, no 1, 70-74 p.
desalting, on-line, preconcentration, biological sample, mass spectrometry, electrophoresis, octadecyltrimethoxysilane, sol-gel
IdentifiersURN: urn:nbn:se:uu:diva-94821DOI: 10.1016/j.jchromb.2006.05.012ISI: 000240712700011PubMedID: 16814621OAI: oai:DiVA.org:uu-94821DiVA: diva2:168812