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Reticulate or treelike chloroplast DNA evolution in Sileneae (Caryophyllaceae)?
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Systematic Botany.
2008 (English)In: Molecular Phylogenetics and Evolution, ISSN 1055-7903, E-ISSN 1095-9513, Vol. 48, no 1, 313-325 p.Article in journal (Refereed) Published
Abstract [en]

Despite sampling of up to 25 kb of chloroplast DNA sequence from 24 species in Sileneae a number of nodes in the phylogeny remain poorly supported and it is not expected that additional sequence sampling will converge to a reliable phylogenetic hypothesis in these parts of the tree. The main reason for this is probably a combination of rapid radiation and substitution rate heterogeneity. Poor resolution among closely related species are often explained by low levels of variation in chloroplast data, but the problem with our data appear to be high levels of homoplasy. Tree-like cpDNA evolution cannot be rejected, but apparent incongruent patterns between different regions are evaluated with the possibility of ancient interspecific chloroplast recombination as explanatory model. However, several major phylogenetic relationships, previously not recognized, are confidently resolved, e.g. the grouping of the two SW Anatolian taxa S. cryptoneura and S. sordida strongly disagrees with previous studies on nuclear DNA sequence data, and indicate a possible case of homoploid hybrid origin. The closely related S. atocioides and S. aegyptiaca form a sister group to Lychnis and the rest of Silene, thus suggesting that Silene may be paraphyletic, despite recent revisions based on molecular data.

Place, publisher, year, edition, pages
2008. Vol. 48, no 1, 313-325 p.
Keyword [en]
Sileneae, cpDNA, chloroplast recombination, incongruence, indel characters
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-94919DOI: 10.1016/j.ympev.2008.04.015ISI: 000257275200027OAI: oai:DiVA.org:uu-94919DiVA: diva2:168940
Available from: 2006-09-28 Created: 2006-09-28 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Phylogenetic Support and Chloroplast Genome Evolution in Sileneae (Caryophyllaceae)
Open this publication in new window or tab >>Phylogenetic Support and Chloroplast Genome Evolution in Sileneae (Caryophyllaceae)
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Evolutionary biology is dependent on accurate phylogenies. In this thesis two branch support methods, Bayesian posterior probablities and bootstrap frequencies, were evaluated with simulated data and empirical data from the chloroplast genome. Bayesian inference was found to be more powerful and less conservative than maximum likelihood bootstrapping, but considerably more sensitive to choice of parameters. Bayesian inference increased in power when data were underparameterized, but the associated increase in type I error was comparatively larger.

The chloroplast DNA phylogeny of the tribe Sileneae (Caryophyllaceae) was inferred by analysis of 33,149 aligned nucleotide bases representing 24 taxa. The position of the SW Anatolian taxa Silene cryptoneura and S. sordida strongly disagreed with previous studies on nuclear DNA sequence data, and indicate a possible case of homoploid hybrid origin. Silene atocioides and S. aegyptiaca formed a sister group to Lychnis and remaining Silene, thus suggesting that Silene may be paraphyletic, despite recent revisions based on molecular data. Several nodes in the phylogeny remained poorly supported, despite large amounts of data. Additional sequence sampling is not expected to solve this problem. The main reason for poor resolution is probably a combination of rapid radiation and substitution rate hererogeneity. Apparent incongruent patterns between different regions of the chloroplast genome are evaluated with ancient interspecific chloroplast recombination as explanatory model.

Extremely elevated substitution rates in the exons of the plastid clpP gene was documented in Oenothera and three separate lineages of Sileneae. Introns have been lost in some of the lineages, but where present, intron sequences have a markedly slower substitution rate, similar to the rates found in other introns of their genomes. Three branches in the phylogeny show significant whole gene positive selection. In two of the lineages multiple partial copies of the gene were found.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 41 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 226
Keyword
Biology, Phylogenetics, Bayesian inference, Bootstrapping, cpDNA, Sileneae, Interspecific chloroplast recombination, Hybridization, clpP, Positive selection, Extreme substitution rates, Biologi
Identifiers
urn:nbn:se:uu:diva-7166 (URN)91-554-6668-0 (ISBN)
Public defence
2006-10-20, Zootis-salen, EBC, Villavägen 9, Uppsala, 09:00 (English)
Opponent
Supervisors
Available from: 2006-09-28 Created: 2006-09-28 Last updated: 2009-04-02Bibliographically approved

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