Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays
2006 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 356, no 1, 132-141 p.Article in journal (Refereed) Published
A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.
Place, publisher, year, edition, pages
2006. Vol. 356, no 1, 132-141 p.
oligonucleotide, synthesis, purification, cartridge, microarray
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-94934DOI: 10.1016/j.ab.2006.05.008ISI: 000240170700017PubMedID: 16769031OAI: oai:DiVA.org:uu-94934DiVA: diva2:168960