Synthesis of RNA Using 2'-O-DTM Protection
2006 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 128, no 38, 12356-12357 p.Article in journal (Refereed) Published
tert-Butyldithiomethyl (DTM), a novel hydroxyl protecting group, cleavable under reductive conditions, was developed and applied for the protection of 2′-OH during solid-phase RNA synthesis. This function is compatible with all standard protecting groups used in oligonucleotide synthesis, and allows for fast and high-yield synthesis of RNA. Oligonucleotides containing the 2′-O-DTM groups can be easily deprotected under the mildest possible aqueous and homogeneous conditions. The preserved 5′-O-DMTr function can be used for high-throughput cartridge RNA purification.
Place, publisher, year, edition, pages
2006. Vol. 128, no 38, 12356-12357 p.
IdentifiersURN: urn:nbn:se:uu:diva-94935DOI: 10.1021/ja0636587ISI: 000240612700003PubMedID: 16984152OAI: oai:DiVA.org:uu-94935DiVA: diva2:168961