Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos
2007 (English)In: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 23, no 1, 63-74 p.Article in journal (Refereed) Published
Apoptosis may be involved in diabetes-induced embryonic dysmorphogenesis. We estimated the occurrence of apoptosis in embryos of a rat model for diabetic pregnancy. We found decreased Bcl-2, increased Bax and cleaved Caspase 3 proteins in embryos from diabetic rats. Moreover, we found increased activation of Caspase 3 in cells from embryos previously exposed to a diabetes-like environment (in vivo, in vitro) compared to cells from control embryos, which was normalized by supplementation of N-acetylcysteine or apoptosis inhibitor. We detected increased propidium iodide uptake in embryonic cells exposed to maternal diabetes, a finding confirmed by vital staining. Additionally, we found increased dysmorphogenesis in embryos exposed to a diabetic environment in vivo and in vitro. Exposure to a diabetic milieu during organogenesis increases apoptosis in embryonic cells and dysmorphogenesis in embryos. Enhanced apoptotic rate may have a role in diabetic embryopathy by inducing disturbed embryonic maturation, increased rates of resorptions and congenital malformations.
Place, publisher, year, edition, pages
2007. Vol. 23, no 1, 63-74 p.
Apoptosis, Bax, Bcl-2, Caspase 3, Diabetes in pregnancy, Embryopathy, Rat
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-95025DOI: 10.1016/j.reprotox.2006.08.009ISI: 000243772800007PubMedID: 17034987OAI: oai:DiVA.org:uu-95025DiVA: diva2:169078