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Functionality and stability of heparin immobilized onto poly(dimethylsiloxane)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
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2005 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 45, no 2, 76-81 p.Article in journal (Refereed) Published
Abstract [en]

Poly(dimethylsiloxane) (PDMS) has become an attractive material when working in the field of microfluidics, mainly because of the rapid prototyping process it involves. The increased surface volume ratio in microchannels makes the interaction between sample and material surface highly important, evident when handling complex biological samples such as plasma or blood. This study demonstrates a new grade of non-covalent heparin surface that adds efficient anticoagulant property to the PDMS material. The surface modification is a simple and fast one-step process performed at neutral pH, optimal when working with closed microsystems. The heparin formed a uniform and functional coating on hydrophobic PDMS with comparatively high level of antithrombin-binding capacity. In addition, long-term studies revelaed that the immobilized heparin was more or less stable in the microchannels over a time of three weeks. Recalcified plasma in contact with native PDMS showed complete coagulation after 1 h, while no fibrin formation was detected in plasma incubated on heparin-coated PDMS within the same time. In conclusion, we see the heparin coating developed and evaluated in this study as a tool that greatly facilitates the use of PDMS in microfluidics dealing with plasma or blood samples.

Place, publisher, year, edition, pages
2005. Vol. 45, no 2, 76-81 p.
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-95124DOI: 10.1016/j.colsurfb.2005.07.004OAI: oai:DiVA.org:uu-95124DiVA: diva2:169215
Available from: 2006-11-17 Created: 2006-11-17 Last updated: 2013-11-08Bibliographically approved
In thesis
1. Microfluidics in Surface Modified PDMS: Towards Miniaturized Diagnostic Tools
Open this publication in new window or tab >>Microfluidics in Surface Modified PDMS: Towards Miniaturized Diagnostic Tools
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

There is a strong trend in fabricating miniaturized total analytical systems, µTAS, for various biochemical and cell biology applications. These miniaturized systems could e.g. gain better separation performances, be faster, consume less expensive reagents and be used for studies that are difficult to access in the macro world. Disposable µTAS eliminate the risk of carry-over and can be fabricated to a low cost.

This work focused on the development of µTAS modules with the intentional use for miniaturized diagnostics. Modules for blood separation, desalting, enrichment, separation and ESI-MS detection were successfully fabricated. Surface coatings were additionally developed and evaluated for applications in µTAS with complex biological samples. The first heparin coating could be easily immobilized in a one-step-process, whereas the second heparin coating was aimed to form a hydrophilic surface that was able to draw blood or plasma samples into a microfluidic system by capillary forces.

The last mentioned heparin surface was further utilized when developing a chip-based sensor for performing CD4-count in human blood, an important marker to determine the stage of an HIV-infection.

All devices in this work were fabricated in PDMS, an elastomeric polymer with the advantage of rapid and less expensive prototyping of the microfabricated master. It was shown that PDMS could be considered as the material of choice for future commercial µTAS. The devices were intentionally produced using a low grade of fabrication complexity. It was however demonstrated that even with low complexity, it is possible to integrate several functional chip modules into a single microfluidic device.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 52 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 241
Keyword
Materials science, µTAS, micro total analysis system, PDMS, poly(dimethylsiloxane), microfluidics, heparin, blood filtration, on-chip, ESI-MS, desalting, QCM-D, biocompatible, CD4, capillary flow, lab-on-chip, microfabrication, enrichment, point-of-care, hydrophilic, oxidation, Materialvetenskap
Identifiers
urn:nbn:se:uu:diva-7270 (URN)91-554-6716-4 (ISBN)
Public defence
2006-12-08, Polhemsalen, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:30
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Supervisors
Available from: 2006-11-17 Created: 2006-11-17Bibliographically approved

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Thorslund, SaraLarsson, RolfNikolajeff, FredrikBergquist, Jonas

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Department of Engineering SciencesDepartment of Oncology, Radiology and Clinical ImmunologyAnalytical Chemistry
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