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Tuneable Peak Deformations in Chiral Liquid Chromatography
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Scientific Computing.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
2007 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, 5838-5847 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2007. Vol. 79, 5838-5847 p.
National Category
Analytical Chemistry Computational Mathematics
Identifiers
URN: urn:nbn:se:uu:diva-95293DOI: 10.1021/ac062330tISI: 000248437700058OAI: oai:DiVA.org:uu-95293DiVA: diva2:169454
Available from: 2006-12-22 Created: 2006-12-22 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Development and Validation of Methods for Characterization of Multi-Component Systems in Preparative LC
Open this publication in new window or tab >>Development and Validation of Methods for Characterization of Multi-Component Systems in Preparative LC
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Utveckling och Validering av Metoder för Karaktärisering av Flerkomponentsystem vid Preparativ Vätskekromatografi
Abstract [en]

This thesis concerns the development and validation of methods for characterization of multi-component preparative LC systems. Measurements of competitive adsorption isotherms are performed to gain detailed information about the interactions inside the chromatography column. This information increases our understanding of the separation process and makes it possible to perform computer simulations and numerical optimizations to find optimal operating conditions.

The methods under focus are called “the tracer-pulse method”, “the inverse method”, and “the inverse method on plateaus”. They are extensions of existing methods, with new experimental and numerical procedures to enable rapid and accurate multi-component adsorption isotherm determination. In the validation it was shown that they can produce results agreeing with traditional methods and that the acquired adsorption isotherm parameters can be used in simulations to accurately predict the outcome of preparative LC separations.

The methods were used to characterize several complex LC systems and two phenomena were discovered and theoretically treated: 1) The presence of invisible deformed peaks in single-component systems. 2) Peak deformations encountered with modern chiral stationary phases, caused by strongly adsorbed eluent additives. The latter type of deformation was highly tuneable and it was possible to adjust the enantiomer peak shapes so that the peaks tailed in opposite directions with the sharp sides in between, yielding baseline resolution at remarkably high sample loads.

In a final applied study both the LC-based perturbation peak method and a biosensor method based on surface plasmon resonance (SPR) were used for the first time for detailed characterization of chiral drug-protein interactions. The fundamental properties of the two very different methods were compared and it was found that the LC method is more suitable for multi-component analysis and that the SPR method is more suitable for stronger interactions.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2006. 48 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 256
Keyword
Analytical chemistry, Liquid chromatography, Preparative LC, Chiral LC, Multi-component LC, Adsorption isotherm, Tracer-pulse method, Inverse method, Inverse method on plateaus, Peak deformations, Chiral drug-protein interactions, Analytisk kemi
Identifiers
urn:nbn:se:uu:diva-7422 (URN)91-554-6762-8 (ISBN)
Public defence
2007-01-19, B22, Biomedicinskt centrum, Husargatan 3, Uppsala, 13:15
Opponent
Supervisors
Available from: 2006-12-22 Created: 2006-12-22Bibliographically approved

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Arnell, RobertFornstedt, Torgny

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