Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
2004 (English)In: Nature Genetics, ISSN 1061-4036, Vol. 36, no 10, 1105-1110 p.Article in journal (Refereed) Published
Chromatin insulators demarcate expression domains by blocking the cis effects of enhancers or silencers in a position-dependent manner1, 2. We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation. Chromatin immunoprecipitation analysis showed that a poly(ADP-ribosyl)ation mark, which exclusively segregates with the maternal allele of the insulator domain in the H19 imprinting control region, requires the bases that are essential for interaction with CTCF3. Chromatin immunoprecipitation−on−chip analysis documented that the link between CTCF and poly(ADP-ribosyl)ation extended to more than 140 mouse CTCF target sites. An insulator trap assay showed that the insulator function of most of these CTCF target sites is sensitive to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase activity. We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions.
Place, publisher, year, edition, pages
2004. Vol. 36, no 10, 1105-1110 p.
IdentifiersURN: urn:nbn:se:uu:diva-95337DOI: 10.1038/ng1426OAI: oai:DiVA.org:uu-95337DiVA: diva2:169511