Impaired antibody responses but normal proliferation of CD4+ T cells in mice lacking complement receptors 1 and 2
2009 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 70, no 2, 77-84 p.Article in journal (Refereed) Published
Severely impaired Ab responses are seen in animals lacking C (complement) factors C2, C3 or C4 as well as CR1/2 (C receptors 1 and 2). The molecular mechanism behind this phenomenon is not understood. One possibility is that C-containing immune complexes are endocytosed via CR2 on B cells and presented to specific CD4(+) T cells, which would then proliferate and provide efficient help to specific B cells. In vitro, B cells can endocytose immune complexes via CR1/2 and present the Ag to T cells. Whether absence of this Ag presenting function in Cr2(-/-) mice (mice lacking CR1/2) explains their low Ab response is unclear. To address this question, Cr2(-/-) and wild type mice were transferred with OVA-specific T cells, obtained from the DO11.10 strain which has a transgenic TCR recognizing an OVA peptide. The animals were subsequently immunized with sheep red blood cells (SRBC) conjugated to OVA. Interestingly, proliferation of the OVA-specific T cells was normal in Cr2(-/-) mice, although their Ab response to both SRBC and OVA was severely impaired. These observations suggest that the impaired Ab response in Cr2(-/-) mice cannot be explained by a lack of appropriate induction of T cell help.
Place, publisher, year, edition, pages
2009. Vol. 70, no 2, 77-84 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-95525DOI: 10.1111/j.1365-3083.2009.02274.xISI: 000268061600001PubMedID: 19630912OAI: oai:DiVA.org:uu-95525DiVA: diva2:169778