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Purification, characterization, and biological compartmentalization of rat fetal antigen 1
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Comparative Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Comparative Medicine. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
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2000 (English)In: Biology of Reproduction, ISSN 0006-3363, E-ISSN 1529-7268, Vol. 63, no 1, 30-33 p.Article in journal (Refereed) Published
Abstract [en]

This study has established the rat as an animal model for the analysis of the biological role of fetal antigen 1 (FA1), a protein previously described in humans and mice. FA1 was purified from rat amniotic fluid by immunospecific affinity chromatography. Immunochemical identity between mouse and rat FA1 was established by crossed tandem immunoelectrophoresis. Molecular size was analyzed by mass spectrometry (33 kDa). The amino acid composition was determined, and the amino acid sequence was analyzed. The overall amino acid composition and sequence of the 28 first N-terminal amino acids were identical to the corresponding parts of rat preadipocyte factor 1 and rat adrenal zone glomerulosa protein. Extensive sequence similarity was found between rat and mouse FA1 (86%) and between rat and human FA1 (82%). The concentration of FA1 in fetal serum, maternal serum, urine, and amniotic fluid in rats was determined using an ELISA. The highest concentrations were found in fetal serum and amniotic fluid around Day 18 of pregnancy. This is the first report on the physicochemical characteristics and compartmentalization of rat FA1.

Place, publisher, year, edition, pages
Society for the Study of Reproduction , 2000. Vol. 63, no 1, 30-33 p.
Keyword [en]
conceptus, developmental biology, pregnancy
National Category
Medical and Health Sciences Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-95638DOI: 10.1095/biolreprod63.1.30OAI: oai:DiVA.org:uu-95638DiVA: diva2:169936
Available from: 2007-04-03 Created: 2007-04-03 Last updated: 2011-11-02Bibliographically approved
In thesis
1. Protein and mRNA Studies of Rat FA1/Pref-1/dlk
Open this publication in new window or tab >>Protein and mRNA Studies of Rat FA1/Pref-1/dlk
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The timing of cell differentiation is important for development and renewal of well functioning organs and tissues. One protein involved in this process is Preadipocyte factor 1 (Pref-1). Most likely, the role of this protein is to maintain cells in an undifferentiated state.

The work presented in this thesis, has employed the rat as an animal model for the studies of Pref-1. Rat models of obesity (Zucker, ZO) and type II diabetes (Goto-Kakizaki, GK) were used to determine metabolic influence on Pref-1 and adipokine mRNA expression in adipose tissues.

The Pref-1 cleavage product was purified from rat amniotic fluid and physicochemically characterised. Concentration of Pref-1 in serum, amniotic fluid and urine was determined by ELISA. Soluble Pref-1 and the compartmentalisation of the protein were highly similar to what had previously been demonstrated in mice and humans.

Immunohistochemistry studies displayed similar staining patterns of Pref-1 in adrenal glands, ovaries and pituitary glands of non-pregnant and pregnant rats. This suggests that pregnancy do not influence the protein expression of Pref-1 in these organs.

In the GK rats, Pref-1 mRNA was altered and a decrease in the visceral compared to subcutaneous adipose depots was demonstrated, in contrast to the ZO rats. Additionally, adiponectin, leptin, IL-6 and TNF-α mRNA levels were altered in the diabetic strain, indicating that this animal model expresses many of the typical features of type II diabetes.

In conclusion, the rat is an appropriate model for studies of FA1/Pref-1/dlk. Pref-1 is highly elevated in fetal and maternal serum during pregnancy. However, the expression of Pref-1 in some endocrine tissues did not alter due to pregnancy. The mRNA expression of Pref-1 was altered between adipose depots and demonstrated to be affected by metabolic disturbances in the animals.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 56 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 244
Keyword
Laboratory animals, Preadipocyte factor 1, delta like protein, Fetal antigen 1, Goto-Kakizaki, Zucker, adipokines, adipose tissue, diabetes, obesity, Försöksdjursvetenskap
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-7773 (URN)978-91-554-6841-5 (ISBN)
Public defence
2007-04-24, B42, BMC, Husargatan 3, Uppsala, 09:15
Opponent
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Available from: 2007-04-03 Created: 2007-04-03 Last updated: 2017-01-19Bibliographically approved

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Carlsson, Hans-ErikPersdotter Hedlund, GabriellaFries, ErikEriksson, Ulf J.

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Carlsson, Hans-ErikPersdotter Hedlund, GabriellaFries, ErikEriksson, Ulf J.
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Department of PhysiologyDepartment of Comparative MedicineDepartment of Medical Biochemistry and MicrobiologyDepartment of Medical Cell Biology
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Biology of Reproduction
Medical and Health SciencesMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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