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Fetal antigen 1 (FA1) in the adult rat adrenal gland, ovary and pituitary gland
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Physiology.
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2003 (English)In: In Vivo, ISSN 0258-851X, E-ISSN 1791-7549, Vol. 17, no 1, 1-4 p.Article in journal (Refereed) Published
Abstract [en]

Fetal antigen 1 (FA1) is a circulating glycoprotein containing six epidermal growth factor (EGF)-like repeats. FA1's larger membrane-bound precursor is defined by the cDNAs referred to as either human delta-like (dlk) or human adrenal specific cDNA, pG2. In rodents FA1 has also been studied under the names of preadipocyte factor 1 (Pref-1), and zona glomerulosa-specific factor (ZOG). FA1 is abundantly expressed in fetal tissues, but in the mature cells of the adult organism the tissue presence of the protein seems to be restricted to neuroendocrine tissues. The present study demonstrates FA1 localisation in endocrine tissues of the adult female rat in which the protein was found present in the medulla and the zona glomerulosa of the cortex of the adrenal glands, in the pars distalis of the adenohypophysis, and in the ovarian granulosa lutein cells. No staining was found in the pancreas, which is in contrast to what has been described in the human.

Place, publisher, year, edition, pages
2003. Vol. 17, no 1, 1-4 p.
Keyword [en]
rat; fetal antigen 1 (FA1); epidermal growth factor (EGF); delta-like cDNA; adrenal-specific cDNA; preadipocyte factor 1 (Pref-1); zona glomerulosa-specific factor (ZOG); localisation; adrenal gland; pituitary gland; hypophysis; ovary
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-95639OAI: oai:DiVA.org:uu-95639DiVA: diva2:169937
Available from: 2007-04-03 Created: 2007-04-03 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Protein and mRNA Studies of Rat FA1/Pref-1/dlk
Open this publication in new window or tab >>Protein and mRNA Studies of Rat FA1/Pref-1/dlk
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The timing of cell differentiation is important for development and renewal of well functioning organs and tissues. One protein involved in this process is Preadipocyte factor 1 (Pref-1). Most likely, the role of this protein is to maintain cells in an undifferentiated state.

The work presented in this thesis, has employed the rat as an animal model for the studies of Pref-1. Rat models of obesity (Zucker, ZO) and type II diabetes (Goto-Kakizaki, GK) were used to determine metabolic influence on Pref-1 and adipokine mRNA expression in adipose tissues.

The Pref-1 cleavage product was purified from rat amniotic fluid and physicochemically characterised. Concentration of Pref-1 in serum, amniotic fluid and urine was determined by ELISA. Soluble Pref-1 and the compartmentalisation of the protein were highly similar to what had previously been demonstrated in mice and humans.

Immunohistochemistry studies displayed similar staining patterns of Pref-1 in adrenal glands, ovaries and pituitary glands of non-pregnant and pregnant rats. This suggests that pregnancy do not influence the protein expression of Pref-1 in these organs.

In the GK rats, Pref-1 mRNA was altered and a decrease in the visceral compared to subcutaneous adipose depots was demonstrated, in contrast to the ZO rats. Additionally, adiponectin, leptin, IL-6 and TNF-α mRNA levels were altered in the diabetic strain, indicating that this animal model expresses many of the typical features of type II diabetes.

In conclusion, the rat is an appropriate model for studies of FA1/Pref-1/dlk. Pref-1 is highly elevated in fetal and maternal serum during pregnancy. However, the expression of Pref-1 in some endocrine tissues did not alter due to pregnancy. The mRNA expression of Pref-1 was altered between adipose depots and demonstrated to be affected by metabolic disturbances in the animals.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2007. 56 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 244
Keyword
Laboratory animals, Preadipocyte factor 1, delta like protein, Fetal antigen 1, Goto-Kakizaki, Zucker, adipokines, adipose tissue, diabetes, obesity, Försöksdjursvetenskap
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:uu:diva-7773 (URN)978-91-554-6841-5 (ISBN)
Public defence
2007-04-24, B42, BMC, Husargatan 3, Uppsala, 09:15
Opponent
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Available from: 2007-04-03 Created: 2007-04-03 Last updated: 2017-01-19Bibliographically approved

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Persdotter Hedlund, Gabriella

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